| 引用本文:郭蓉，高蔚，殷茵，韩雪珍，陈香云，刘甜甜，吴丹桐，安致君，杨桢，刘珍洪.温中祛寒三方对急性胰腺炎模型小鼠TRPV1表达的影响[J].世界中医药,2020,(15):. |
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| 温中祛寒三方对急性胰腺炎模型小鼠TRPV1表达的影响 |
| Effects of 3 Formulas of Warming Spleen and Stomach for Dispelling Cold on the Expression of TRPV1 in Model Mice with Acute Pancreatitis |
| 投稿时间：2019-07-26 |
| DOI：10.3969/j.issn.1673-7202.2020.15.013 |
| 中文关键词: 小建中汤 吴茱萸汤 理中丸 急性胰腺炎 热敏通道 瞬时受体电位香草酸受体1 神经激肽1受体 降钙素基因相关肽 作用机制 |
| English Keywords:Xiaojianzhong Decoction Wuzhuyu Decoction Lizhong Pills Acute pancreatitis Thermal channel TRPV1 NK1R CGRP Mechanism |
| 基金项目:国家自然科学基金面上项目（81573847） |
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| 中文摘要: |
| 目的:探讨温中祛寒的小建中汤、吴茱萸汤和理中丸减轻急性胰腺炎炎性反应和疼痛的作用机制。方法:选取30只雄性小鼠并随机分为正常组、模型组、小建中汤组、吴茱萸汤组、理中丸组，每组6只。模型组和各给药组小鼠使用雨蛙素造模。观察组分别灌胃小建中、吴茱萸汤和理中丸水溶液。正常组和模型组小鼠给予等量双蒸水灌胃。检测并比较各组小鼠疼痛行为、血清淀粉酶（AMY）、髓过氧化物酶（MPO）、胰腺病理及胰腺组织中瞬时受体电位香草酸受体1（TRPV1）、NK1R和降钙素基因相关肽（CGRP）蛋白表达。结果:与模型组比较，小建中汤、吴茱萸汤和理中丸均能延迟小鼠疼痛行为第1次出现时间、降低MPO活性、降低AMY水平，差异有统计学意义（P<0.05），下调TRPV1、NK1R和CGRP蛋白表达，差异有统计学意义（P<0.05）。结论:温中祛寒三方减轻急性胰腺炎小鼠模型的炎性反应和疼痛可能是通过下调TRPV1、NK1R和CGRP的表达来实现。 |
| English Summary: |
| To explore the mechanism Xiaojianzhong Decoction,Wuzhuyu Decoction and Lizhong Pills in the formulas of warming spleen and stomach for dispelling cold in alleviating inflammation and pain in acute pancreatitis.Methods:A total of 30 male mice were randomly divided into a normal group,a model group,a Xiaojianzhong Decoction group,a Wuzhuyu Decoction group and a Lizhong Pills group,with 6 mice in each group.Mice in the model group and each administration group were modeled with caerulein.Mice in the treatment groups were given intragastric solution of Xiaojianzhong Decoction,Wuzhuyu Decoction and Lizhong Pills respectively.Mice in the normal group and the model group were given the same amount of double distilled water by gavage.The pain behavior,AMY,MPO,protein expression of TRPV1,NK1R and CGRP in pancreatic pathology and tissues of mice in each group were detected and compared.Results:Compared with the model group,Xiaojianzhong Decoction,Wuzhuyu Decoction and Lizhong Pills can delay the first occurrence time of pain behavior in mice,reduce MPO activity ,reduce AMY level (P<0.05),and down-regulate the expression of TRPV1,NK1R and CGRP proteins (P<0.05).Conclusion:The 3 formulas of warming spleen and stomach for dispelling cold can alleviate inflammation and pain in acute pancreatitis model mice by down-regulating the expression of TRPV1,NK1R and CGRP proteins. |
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