To study the efficacy and safety of Longmu Zhuanggu Granules in the prevention and treatment of vitamin D deficiency rickets in infantile.Methods:A total of 96 healthy newborn babies admitted to Wuhan Children's Hospital，Tongji Medical College，Huazhong University of Science & Technology from May 2017 to May 2018 were selected as the research subjects and were randomly divided into an observation group and a control group according to the random number table method，with 48 cases in each group.The control group was given Vitamin A and D Drops for prevention and treatment，while the observation group was given Longmu Zhuanggu Granules for prevention and treatment.After 12 months of continuous treatment，the incidence of vitamin D deficiency rickets，blood biochemical indicators，bone examination，total score of TCM symptoms，follow-up and safety were compared between the 2 groups.Results:The morbidity of vitamin D deficiency rickets in the observation group was lower than that in the control group.There was a significant difference between the 2 groups(P<0.05).After treatment，25-(OH)D3 and calcium phosphate product in the observation group were higher than those in the control group，while serum alkaline phosphatase(ALP)was lower than that in the control group，and the difference between the 2 groups was statistically significant(P<0.05).After 3，6，9 and 12 months follow-up observation，the total score of TCM symptoms in the observation group was lower than that in the control group，and the difference between the 2 groups was statistically significant(P<0.05).The incidence of adverse reactions in the observation group was lower than that in the control group.There was a significant difference between the 2 groups(P<0.05).Conclusion:Longmu Zhuanggu Granules can be used for the prevention and treatment of infantile rickets with vitamin D deficiency.It can significantly lower the morbidity，serological indicators and TCM syndrome score etc and has less adverse effects，which is worthy of clinical application.