To explore the renal protective effects of Tangshen Decoction on type 2 diabetic nephropathy(DN)rats.Methods:A total of 60 type 2 diabetic nephropathy rats with deficiency of both qi and yin were established by single nephrectomy，high fat diet and small dose of streptozotocin(STZ)intraperitoneal injection combined with behavioral intervention(hunger and satiety disorder，excessive work and rest，fright)and were randomly divided into a group A(sham operation)，a group B(model)，a group C(Tangshen Decoction)and a group D(benazepril)，with 15 rats in each group.Fasting blood glucose(FBG)，2 h postprandial blood glucose(PBG)，urea nitrogen(BUN)，serum creatinine(CR)，24-hour urinary albumin(24 h UAlb)，Cystatin C(Cys-C)，blood and urine β 2-microglobulin(β 2-mg)were detected after intervention.The pathological characteristics of renal tissue were compared between the 2 groups.Results:Compared with group A，BUN and Cr in group B did not increase significantly(P>0.05)，FBG，PBG，24 h-UAlb，Cys-c，blood and urine β2-MG increased significantly(P<0.05); compared with group B，24 h-UAlb，Cys-c，blood and urine β2-MG in group C and D decreased significantly(P<0.05); the above indexes in group C and group D had no statistical significance(P>0.05); renal tissue lesions from normal to severe were A group，C group，D group，B group.Conclusion:Tangshen Decoction，the Chinese patent medicine can effectively reduce blood sugar，24 h-UAlb，Cys-c，blood and urine group β2-MG in type 2 diabetic nephropathy rats，and has protective effects on early DN kidney.