| 引用本文:薛静波1，陶艳艳1，刘成海1，2，3，4.扶正化瘀方体外给药方法及生物活性差异的探讨[J].世界中医药,2020,(19):. |
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| 扶正化瘀方体外给药方法及生物活性差异的探讨 |
| Exploration of Fuzheng Huayu Formula in-Vitro Administration Method and Bio-Activity Differences |
| 投稿时间：2020-09-10 |
| DOI：10.3969/j.issn.1673-7202.2020.19.002 |
| 中文关键词: 中药质量控制 生物活性 扶正化瘀方 抗肝纤维化 体外给药 |
| English Keywords:Traditional Chinese medicine quality control Bioactivity Fuzheng Huayu Formula Anti hepatic fibrosis In-vitro administration medicine |
| 基金项目:国家自然科学基金委员会重点项目(81730109) |
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| 中文摘要: |
| 目的:建立扶正化瘀方体外给药方法,观察不同批次间药效差异,为中药复方生物活性的质控奠定基础。方法:选择人肝细胞L-02、小鼠单核巨噬细胞RAW264.7和小鼠原代骨髓来源巨噬细胞(Bone Marrow-derived Macrophage,BMDM),分别建立肝细胞过氧化损伤模型和巨噬细胞活化模型,首先通过考察溶媒、作用浓度等因素对细胞效应的影响，基于扶正化瘀体外给药的最佳保肝抗炎效果，建立扶正化瘀浸膏体外配置给药方法；再比较不同批次的扶正化瘀浸膏对肝细胞损伤和巨噬细胞活化药效差异。结果:扶正化瘀浸膏以二甲基亚枫溶解不过滤,不同批次扶正化瘀的化学指标成分不同,但在细胞生物活性总体无显著性差异,其中批号171005与其他批次的部分生物活性略有不同。结论:确立了扶正化瘀方体外给药方法，发现其化学成分质控与细胞生物活性不一致。 |
| English Summary: |
| To establish in-vitro administration method for Fuzheng Huayu(FZHY) and further to observe the medicinal effect differences of different batches, which will be fundermental for establishment of Traditional Chinese Medicine(TCM) bioactivity-based quality control. Methods:Human hepatocyte cell L-02, mouse leukemia cell of monocyte macrophage Raw264.7, mouse bone marrow-derived macrophage (BMDM) were selected to establish hepatocyte peroxidation injury model and macrophage activation model. Firstly, by investigating the influence of factors such as solvent, concentration and other factors on cellular effects, based on the best hepatoprotective and anti-inflammatory effects of Fuzheng Huayu in vitro medicine, an in vitro drug delivery method for Fuzheng Huayu extract was established; the effect of Fuzheng Huayu extract on liver cell damage and macrophage activation was different. Results:Fuzheng Huayu extract was dissolved in dimethyl sulfoxide and was not filtered. The chemical index components of different batches of Fuzheng Huayu are different, but there is no significant difference in overall cell biological activity. Among them, the biological activity of batch number 171005 was slightly less than that of other batches and was a little different. Conclusion:Hereby, standard in-vitro FZHY administration method was established. We found chemical component-based quality control is not consisted with its cellular bioactivity. |
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