| 引用本文:纪佳灏，黄作阵.电针对肠易激综合征内脏痛大鼠β内啡肽及脑啡肽的调节作用[J].世界中医药,2020,(19):. |
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| 电针对肠易激综合征内脏痛大鼠β内啡肽及脑啡肽的调节作用 |
| Study on the Regulating Effects of Electroacupuncture on β-Endorphin and Enkephalin in Visceral Pain Rats with Irritable Bowel Syndrome |
| 投稿时间：2019-03-19 |
| DOI：10.3969/j.issn.1673-7202.2020.19.008 |
| 中文关键词: 电针 肠易激综合征 内脏痛 大鼠 β-内啡肽 脑啡肽 天枢 上巨虚 |
| English Keywords:Electroacupuncture Irritable bowel syndrome Visceral pain Rats β-endorphin Enkephalin Tianshu(S25) Shangjuxu(S37) |
| 基金项目:北京中医药大学创新团队（2011-CXTD-06）；北京市共建项目（0101186） |
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| 中文摘要: |
| 目的：观察电针对肠易激综合征(IBS)内脏痛大鼠血清及下丘脑β-内啡肽（β-EP）及脑啡肽（ENK）的调节作用，探讨电针对IBS内脏痛的镇痛效应。方法：制备IBS内脏痛大鼠模型。实验分为模型组、电针组、假电针组和正常组。取天枢和上巨虚穴（双侧）为针刺穴位，针刺后连接电针仪；假电针组进针透皮即止，连接电针仪但不通电。模型组和正常组做与电针组相同的固定，不进行任何治疗。治疗后测试各组大鼠AWR评分，检测血清及下丘脑β-EP及ENK水平。结果：与正常组比较，模型组大鼠AWR评分在各个压力梯度下均显著升高、血清β-EP及ENK水平显著降低，差异有统计学意义（P<0.05），下丘脑β-EP、ENK水平差异无统计学意义（P>0.05）。与模型组、假电针组比较，电针组大鼠AWR评分明显降低，差异有统计学意义（P<0.05）；血清和下丘脑β-EP、ENK水平显著增高，差异有统计学意义（P<0.05）。与模型组比较，假电针组大鼠AWR评分、血清和下丘脑β-EP、ENK水平差异无统计学意义（P>0.05）。结论：电针有效缓解IBS大鼠内脏痛，其作用机制可能与调节血清和下丘脑β-EP、ENK的水平有关。 |
| English Summary: |
| To observe the regulatory effects of electroacupuncture on serum and hypothalamic β-endorphin(β-EP)and enkephalin(ENK)in rats with IBS visceral pain,and to explore the analgesic effect of electroacupuncture on IBS visceral pain.Methods:The rat model of IBS visceral pain was prepared.The experiment was divided into a model group,an electroacupuncture group,a sham electroacupuncture group and a normal group.Tianshu(S25)and Shangjuxu(S37)acupoints(both sides)was taken as acupuncture points,and the electroacupuncture instrument after acupuncture was connected.The sham electro-acupuncture group stopped when the needle was inserted through the skin,and the electro-acupuncture device was connected but not powered.The model group and the normal group received the same fixation as the electro-acupuncture group without any treatment.After treatment,the AWR scores of each group of rats were tested,and the serum and hypothalamic β-EP and ENK contents were detected by ELISA.Results:Compared with the normal group,the AWR score of the model group was significantly increased under each pressure gradient,the serum β-EP and ENK content were significantly reduced(all P<0.01),and the hypothalamic β-EP and ENK content did not change significantly(P>0.05).Compared with the model group and the sham electroacupuncture group,the AWR score of the rats in the electroacupuncture group was significantly reduced(P<0.05); the serum and hypothalamic β-EP and ENK levels were significantly increased(P<0.01,P<0.05).Compared with the model group,there were no significant changes in the AWR score,serum and hypothalamic β-EP,ENK content of rats in the sham electroacupuncture group(P>0.05).Conclusion:Electroacupuncture can effectively relieve visceral pain in IBS rats,and its mechanism may be related to the regulation of the contents of β-EP and ENK in serum and hypothalamus. |
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