| 引用本文:杜玉青，刘亚莉，李友山，刘凤桐，黄天一.三七、黄芪总皂苷联合骨髓干细胞移植对糖尿病溃疡miRNA-146a的影响[J].世界中医药,2020,(19):. |
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| 三七、黄芪总皂苷联合骨髓干细胞移植对糖尿病溃疡miRNA-146a的影响 |
| Effects of Panax Notoginseng and Astragalus Saponin Combined with Autologous Bone Marrow Stem Cell Transplantation on miRNA-146a in Diabetic Ulcer |
| 投稿时间：2019-07-22 |
| DOI：10.3969/j.issn.1673-7202.2020.19.010 |
| 中文关键词: 三七 黄芪 大鼠 糖尿病溃疡 自体骨髓干细胞移植 |
| English Keywords:Radix Notoginseng Radix Astragali seu Hedysari Rats Diabetic ulcer Autologous bone marrow stem cell transplantation |
| 基金项目:2016年度北京中医药大学基本科研业务费项目(2016-JYB-JSPY-018)；北京中医药大学东直门医院2017“青苗人才”计划项目（DZMYS-201701） |
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| 中文摘要: |
| 目的：探讨三七总皂苷(PNS)、黄芪总皂苷（TSA）联合自体骨髓干细胞治疗糖尿病溃疡对miRNA-146a的影响。方法：选取SD大鼠60只，在双足背上制成糖尿病足模型，采用全骨髓培养加贴壁法分离、扩增至第4代的骨髓间充质干细胞,按随机数字法将大鼠分为空白组、对照组、干细胞组、黄芪组；三七组干细胞组、黄芪组、三七组创面每日注射自体骨髓干细胞(0.3 mL/cm2)，黄芪组每日加注黄芪注射液(0.3 mL/cm2)，三七组每日加注血塞通(0.4 mL/cm2)，7 d后处死，采用PCR法检测miRNA-146a水平，Western blot法检测TLR4 miRNA、NOD1 miRNA、核因子-κB miRNA的表达。结果：与对照组比较，干细胞组、黄芪组和三七组大鼠创面愈合率极显著增大，差异有统计学意义（P<0.05）；与对照组比较，干细胞组、黄芪组和三七组大鼠miRNA-146a、TLR4 miRNA、核因子-κB miRNA、NOD1 miRNA表达量显著增大，差异有统计学意义（P<0.05）；与干细胞组比较，三七组miRNA-146a、TLR4 miRNA、核因子-κB miRNA、NOD1 miRNA表达量显著增大，差异有统计学意义（P<0.05）。结论：三七总皂苷、黄芪总皂苷联合骨髓干细胞治疗糖尿病足溃疡有效，其愈合机制可能与TLR4/核因子-κB通路上调miRNA-146a的表达有关。 |
| English Summary: |
| To explore the mechanism of panax notoginseng saponins，total saponins of Astragalus combined with autologous bone marrow stem cell transplantation for treating diabetic ulcer on mirna-146a.Methods:A total of 60 SD rats were selected to make diabetic foot model on the dorsum of both feet.The fourth generation of bone marrow mesenchymal stem cells were isolated and expanded by whole bone marrow culture and adherent method.According random number table method，the rats were randomly divided into a blank group，a control group，a stem cell group，and Radix Astragali seu Hedysar group.The wounds of Radix Notoginseng，stem cell group，Radix Astragali seu Hedysar group were injected with autologous bone marrow stem cells(0.3 mL/CM2)every day，Radix Astragali seu Hedysar group was injected with astragalus injection(0.3 mL/CM2)every day，and Radix Notoginseng group was injected with Xuesaitong(0.4 mL/CM2)every day.After 7 days，they were sacrificed.The content of miRNA-146a was detected by PCR.The expression of TLR4 miRNA，NOD1 miRNA，and NF-κB miRNA was detected by Western blot.Results:Compared with the control group，the wound healing rate of rats in stem cell group，Radix Astragali seu Hedysar group and Radix Notoginseng group increased significantly(P<0.01)，and the expression of miRNA-146a，TLR4 miRNA，NF-κ B miRNA and NOD1 miRNA in stem cell group，Radix Astragali seu Hedysari group and Radix Notoginseng group increased significantly compared with the control group(P<0.05).Compared with stem cell group，the expression of miRNA-146a，TLR4 miRNA，NF-κ B miRNA and NOD1 miRNA in Radix Notoginseng group was significantly increased(P<0.05).Conclusion:Panax notoginseng saponins and astragalus saponins combined with bone marrow stem cells are effective in the treatment of diabetic foot ulcer，and the healing mechanism may be related to the up-regulation of miRNA-146a expression by TLR4/NF-κ B pathway. |
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