To explore the regulation of acupoint intensive catgut embedding therapy on ICC cells and neurons in rectal tissue of slow transit constipation (STC). Methods:A total of 41 STC patients and 41 non-constipation patients were selected as the observation group and control group, respectively. The patients in treatment group were given acupoint intensive catgut embedding therapy, while the control group did not receive any constipation related treatment. The morphological of ICC and the expression of SCF and c-kit on RNA were compared before and after treatment in the treatment group. Results:The number of ICC in the rectum tissue increased with the normal morphology after treatment. Compared with the control group, both SCF mRNA and c-kit mRNA in the rectum were significantly lower without treatment (P<0.05, P<0.01). The expression in rectal tissue after treatment was higher than before treatment (P<0.01). Before treatment, the number of neuronal apoptosis in the rectal tissue of the observation group was more than that of the control group (P<0.01). After treatment, the apoptotic cells in the rectal tissue of the patients were significantly reduced, which was significantly different from the control group (P<0.05). Conclusion:Acupoint intensive catgut embedding therapy can improve the injury of ICC cells in rectal tissues of STC patients, reduce the apoptosis of neuron cells and improve the symptoms of constipation.