| 引用本文:毛鑫1，徐玉辉1，姚荣妹1,2，张海江3，崔晓兰1.王不留行治疗尿路感染有效部位筛选及其对膀胱IL-1β的影响[J].世界中医药,2020,(20):. |
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| 王不留行治疗尿路感染有效部位筛选及其对膀胱IL-1β的影响 |
| Effects of Vaccaria segetalis on the Treatment of Effective Part of Urinary Tract Infections and Its Influence on Bladder IL-1β |
| 投稿时间：2019-05-21 |
| DOI：10.3969/j.issn.1673-7202.2020.20.005 |
| 中文关键词: 尿路感染 王不留行 多糖 白细胞介素-1β 大肠杆菌 膀胱炎 有效部位 水提醇沉 |
| English Keywords:Urinary tract infection Vaccaria segetalis Polysaccharide Interleukin-1β Escherichia coli Cystitis Effective part Water extraction and alcohol precipitation |
| 基金项目:国家科技重大专项（2019ZX09721001-005-001） |
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| 中文摘要: |
| 目的：筛选王不留行治疗尿路感染的有效部位，并研究有效部位对膀胱组织IL-1β的影响。方法：选用SD大鼠，以膀胱注射大肠杆菌标准株（ATCC 25922）的方法建立尿路感染模型，大鼠造模后给予王不留行醇溶部位及醇沉部位，给药5 d后通过检测尿液中的细菌数及膀胱组织病理学染色评价其有效性；取有效部位给药后的膀胱组织，检测组织中白细胞介素-1β（IL-1β）mRNA及蛋白的表达。结果：王不留行醇沉部位可明显降低大鼠尿液细菌阳性率，减轻膀胱组织炎性浸润，可明显降低大鼠膀胱组织中IL-1β mRNA及蛋白的表达。王不留行醇沉部位的主要成分为多糖。结论：王不留行多糖提取物为其治疗尿路感染的有效部位。 |
| English Summary: |
| To screen the effective parts of Vaccaria segetalis in the treatment of urinary tract infection,and and to study the effects of effective parts on IL-1β of bladder tissue.Methods:SD rats were used to inject the standard strain of Escherichia coli(ATCC 25922)into the bladder to establish a urinary tract infection model.After the rats were modeled,they were given the alcohol-soluble part and the alcohol-precipitated part of Vaccaria segetalis.The number of bacteria in urine and histopathological staining of the bladder were detected to evaluate its effectiveness; the bladder tissue after administration of the effective part was taken to detect the expression of interleukin-1β(IL-1β)mRNA and protein in the tissue.Results:Vaccaria segetalis's alcohol precipitation site can significantly reduce the positive rate of urine bacteria in rats,and the inflammatory infiltration of bladder tissue,and can significantly reduce the expression of IL-1β mRNA and protein in rat bladder tissue.The main component of Vaccaria segetalis's alcohol precipitation site is polysaccharide.Conclusion:Vaccaria segetalis polysaccharide extract is an effective part of treating urinary tract infection. |
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