To study the optimal concentration of Astragaloside IV(AS-IV)on the proliferation of human endothelial progenitor cells(EPCs)impaired by high glucose,and to explore the biological effects of AS-IV on high glucose impaired EPCs.Methods:Full-term neonatal umbilical cord blood was isolated,cultured,and EPCs were identified.The identified EPCs were pretreated with 30 mmol/L glucose for 120 hours,and were randomly divided into an experimental group(HG+AS-IV group)and a control group(HG Group),while the normal group(NC group)was set.CCK8 was used to detect the proliferation of EPCs at different concentrations of AS-IV(0,25,50,100,200,400 mg/L)after 24 hours,and the proliferation curve was drawn to obtain the preliminary conclusion that AS-IV interfered with the proliferation of EPCs in patients with high glucose damage.Optimal concentration.Furthermore,the effects of the optimal concentration of AS-IV on the proliferation,adhesion,migration,and tube-forming function of high-glucose-damaged EPCs were tested by CCK-8 proliferation assay,adhesion ability test,cell scratch test,and Matrigel in vitro angiogenesis experiment.Results:The optimal concentration of AS-IV to promote the proliferation of high-glucose-damaged EPCs was 100 mg/L; compared with the control group,100 mg/L AS-IV-treated high-glucose-damaged EPCs had the proliferation capacity,the number of adherent cells,and cell migration The rate and the number of tube formation in vitro were significantly increased,and the differences were statistically significant(P1<0.05).At the same time,there was no significant difference between the experimental group and the normal group(P2>0.05).Conclusion:AS-IV can significantly improve the biological function of human EPCs with impaired high glucose in vitro,restore their original vitality and have the potential to mediate angiogenesis.