| 引用本文:余亦程1，熊武2，蔡昫2，王禹萌1，肖慧1，白雪1，邹晓玲1.黄芪甲苷改善高糖受损内皮祖细胞生物学功能的实验研究[J].世界中医药,2020,(21):. |
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| 黄芪甲苷改善高糖受损内皮祖细胞生物学功能的实验研究 |
| Experimental Study of Astragaloside IV on Improving Biological Function of High Glucose Damaged Endothelial Progenitor Cells |
| 投稿时间：2020-02-19 |
| DOI：10.3969/j.issn.1673-7202.2020.21.009 |
| 中文关键词: 黄芪甲苷 内皮祖细胞 生物学功能 体外实验 |
| English Keywords:Astragaloside IV Endothelial progenitor cells Biological function In vitro experiment |
| 基金项目:国家自然科学基金青年科学基金项目(81904217);湖南省卫生计生委科研计划课题项目(B20180739) |
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| 中文摘要: |
| 目的:研究黄芪甲苷(Astragaloside IV,AS-IV)干预高糖受损人内皮祖细胞(Endothelial Progenitor Cells,EPCs)增殖的最佳浓度,并探讨AS-IV对高糖受损EPCs生物学功能的影响。方法:取足月新生儿脐带血分离、培养并鉴定EPCs,将鉴定成功的EPCs用30 mmol/L的葡萄糖预处理120 h后,随机分为实验组(HG+AS-IV组)和对照组(HG组),同时设置正常组(NC组)。用CCK8检测不同浓度AS-IV(0、25、50、100、200、400 mg/L)干预EPCs 24 h后的增殖情况,绘制增殖曲线,初步得出AS-IV干预高糖受损人EPCs增殖的最佳浓度。进而通过CCK-8增殖实验、黏附能力测定试验、细胞划痕试验及Matrigel体外成血管实验检测最佳浓度的AS-IV对高糖受损EPCs增殖、黏附、迁移和成管功能的影响。结果:AS-IV促高糖受损EPCs增殖的最佳浓度为100 mg/L;与对照组比较,100 mg/L AS-IV干预后的高糖受损EPCs增殖能力、黏附细胞数、细胞迁移率、体外成管数均明显增加,差异均有统计学意义(P1<0.05);同时检测实验组与正常组差异无统计学意义(P2>0.05)。结论:AS-IV可显著改善体外高糖受损的人EPCs的生物学功能,恢复其原有活力并具有介导血管新生的潜能。 |
| English Summary: |
| To study the optimal concentration of Astragaloside IV(AS-IV)on the proliferation of human endothelial progenitor cells(EPCs)impaired by high glucose,and to explore the biological effects of AS-IV on high glucose impaired EPCs.Methods:Full-term neonatal umbilical cord blood was isolated,cultured,and EPCs were identified.The identified EPCs were pretreated with 30 mmol/L glucose for 120 hours,and were randomly divided into an experimental group(HG+AS-IV group)and a control group(HG Group),while the normal group(NC group)was set.CCK8 was used to detect the proliferation of EPCs at different concentrations of AS-IV(0,25,50,100,200,400 mg/L)after 24 hours,and the proliferation curve was drawn to obtain the preliminary conclusion that AS-IV interfered with the proliferation of EPCs in patients with high glucose damage.Optimal concentration.Furthermore,the effects of the optimal concentration of AS-IV on the proliferation,adhesion,migration,and tube-forming function of high-glucose-damaged EPCs were tested by CCK-8 proliferation assay,adhesion ability test,cell scratch test,and Matrigel in vitro angiogenesis experiment.Results:The optimal concentration of AS-IV to promote the proliferation of high-glucose-damaged EPCs was 100 mg/L; compared with the control group,100 mg/L AS-IV-treated high-glucose-damaged EPCs had the proliferation capacity,the number of adherent cells,and cell migration The rate and the number of tube formation in vitro were significantly increased,and the differences were statistically significant(P1<0.05).At the same time,there was no significant difference between the experimental group and the normal group(P2>0.05).Conclusion:AS-IV can significantly improve the biological function of human EPCs with impaired high glucose in vitro,restore their original vitality and have the potential to mediate angiogenesis. |
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