| To explore the mechanism of Xiaoxianxiong Decoction in treating of hypertension based on network pharmacology.Methods:The effective chemical constituents and targets of Xiaoxianxiong Decoction were obtained from three databases:SymMap,TCMSP,DrugBank.The targets of hypertension collected by TCMSP,TCMIP and DrugBank databases were compared to the targets of Xiaoxianxiong Decoction to obtain the targets of Xiaoxianxiong Decoction in treating of hypertension.The David database was used to analyze the GO and KEGG pathway enrichment analysis of the mutual target proteins of Xiaoxianxiong Decoction and hypertension.The STRING database was used to construct the PPI network of Xiaoxianxiong Decoction in treating of hypertension.The Cytoscape was used to perform the topological analysis of the PPI network to obtain the key targets.Results:There were 33 active components of Xiaoxianxiong Decoction in treatment of hypertension,48 related targets and 14 key target genes.GO enrichment analysis showed that the biological processes of Xiaoxianxiong Decoction in treatment of hypertension were significantly enriched on the response to abiotic stimulus,muscarinic acetylcholine receptor signaling pathway,regulation of systemic processes,second-messenger-mediated signaling,and cell proliferation etc.Cell components were mainly concentrated on axons,plasma membrane part,asymmetric synapse,nerve terminal,neuron projection,postsynaptic membrane,extracellular matrix etc.Molecular functions were mainly concentrated on amine receptor activity,adrenoceptor activity,muscarinic acetylcholine receptor activity,G-protein coupled acetylcholine receptor activity,alpha-adrenergic receptor activity etc.KEGG pathway enrichment analysis showed that affected pathways included neuroactive ligand-receptor interaction,calcium signaling pathway,vascular smooth muscle contraction,regulation of actin cytoskeleton,tryptophan metabolism etc.Conclusion:The mechanism of Xiaoxianxiong Decoction in treating hypertension may resist inflammatory and oxidative stress and improve endothelial function by quercetin,berberine,R-Hydrogenated berberine of golden thread,cavidine,stigmasterol,coniferin of pinellia tuber and spinasterol of snakegourd fruit,etc.The mechanism of reducing blood pressure may also be through regulating gene expression of related target proteins,regulating vascular tissue remodeling and extracellular matrix metabolism,reducing inflammatory responses,participating in neuroactive ligand-receptor interactions,calcium signaling pathways,and vascular smooth muscle. |
