To investigate the mechanism of diabetes mellitus(DM)treatment by drug pair containing Rhizoma Anemarrhenae and Cortex Phellodendri.Methods:The chemical constituents and targets of Rhizoma Anemarrhenae and Cortex Phellodendri was searched through TCMSP and chemical professional databases，and target and gene conversion were performed in the UniProt database，and existing DM related genes were checked through OMIM and Drugbank databases.Then Cytoscape 3.7.1 was used to construct a component-target network，a target interaction(PPI)network and perform network topology analysis.Finally，Omicshare was used to perform gene GO function enrichment analysis and KEGG pathway enrichment analysis to study its mechanism of action.Results:The compound-target network contained 127 compounds and 107 targets，and key compounds involved quercetin，stigmasterol，kaempferol，etc.The PPI network contained 107 proteins，and key target proteins involved in INS，AKT1，IL6，VEGFA，TNF，etc.The functional enrichment analysis of GO obtained 2 612 items(P<0.05)，of which there were 2 125 biological process(BP)items，138 molecular function(CC)items，and 349 cell composition(MF)items.There were 157 signaling pathways(P<0.05)，involving MAPK signaling pathway，fluid shear stress and atherosclerosis，AGE-RAGE signaling pathway，and insulin resistance and other signal pathways.Conclusion:The main therapeutic active compound of diabetes in Rhizoma Anemarrhenae and Cortex Phellodendri are quercetin，stigmasterol and kaempferol.The targets mainly involve INS，AKT1，IL6，VEGFA，TNF，etc.Through the MAPK signaling pathway，AGE-RAGE signaling pathway，insulin resistance and other signaling pathways，DM is co treated.s