| 引用本文:袁仁智1,2，唐鹏1,2，雍志强1,2，肖卫琼1，康开彪2，潘文2.牛膝干预肾虚血瘀型多囊卵巢综合征的成分靶点和通路分析[J].世界中医药,2020,(22):. |
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| 牛膝干预肾虚血瘀型多囊卵巢综合征的成分靶点和通路分析 |
| Analysis of Component Targets and Pathways of Achyranthes Bidentata Intervention in Renal Deficiency and Blood Stasis Type Polycystic Ovary Syndrome |
| 投稿时间：2019-08-22 |
| DOI：10.3969/j.issn.1673-7202.2020.22.003 |
| 中文关键词: 牛膝 多囊卵巢综合征 肾虚血瘀 网络药理学 成分 靶点 通路 富集分析 |
| English Keywords:Achyranthes bidentata PCOS Kidney deficiency and blood stasis Network pharmacology Components Targets Pathways Enrichment analysis |
| 基金项目:国家自然科学基金项目（81660803） |
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| 中文摘要: |
| 目的：运用网络药理学方法和技术研究牛膝治疗多囊卵巢综合征（PCOS）的主要活性成分、靶点、通路等药理学作用机制。方法：通过中药系统药理学分析平台(TCMSP)筛选出牛膝的活性成分和潜在靶点；查询NCBI数据库获得PCOS疾病靶点，找到药物和疾病的共靶点；通过DAVID数据库对共靶点进行基因功能GO富集分析和KEGG富集分析；采用Cytoscape软件构建成分-靶标-通路网络。结果：筛选得到有靶点的牛膝候选活性化合物共24个，作用于128个靶点；与PCOS的22个共靶点主要涉及AGE-RAGE信号通路、核因子-κB信号通路、TNF信号通路等；共靶点涉及化合物23个，7个靶点涉及5个及以上化合物，ESR1和AR涉及化合物多达19个，还有GSK3B（12个）、ESR2（11个）、PPARG（11个）、ADRB2（9个）涉及较多化合物。结论：牛膝治疗PCOS具有多成分、多靶点协同作用的特点，通过干预分子网络、生物过程和信号通路来发挥抗炎、抗肿瘤、调节血脂、促进微循环及改善胰岛素抵抗的作用。 |
| English Summary: |
| To study the pharmacological mechanism of Achyranthes bidentata in the treatment of polycystic ovary syndrome(PCOS)by network pharmacological methods and techniques.Methods:The active components and potential targets of Achyranthes bidentata were screened by the platform of systematic pharmacological analysis of traditional Chinese medicine(TCMSP); the PCOS disease targets were obtained by querying the NCBI database to find the co-target of drugs and diseases; the gene function GO enrichment analysis and KEGG enrichment analysis were carried out by DAVID database on the co-target database; and the component-target-pathway network was constructed by Cytoscape software.Results:A total of 24 bull knee candidate active compounds were screened，and 128 targets were selected.The 22 common targets with PCOS mainly involved the AGE-RAGE signal path，NF-κB signal path，TNF signal path，etc.A total of 23 target involved in compounds，7 targets involved 5 or more compounds，ESR1 and AR involved as many as 19 compounds，as well as GSK3B(12)，ESR2(11)，PPARG(11)，ADRB2(9)or more compounds.Conclusion:Achyranthes bidentata in the treatment PCOS has the characteristics of multi-component and multi-target synergistic effects.It can play the role of anti-inflammatory，anti-tumor，regulating blood lipid，promoting microcirculation and improving insulin resistance by interfering with molecular network，biological process and signaling pathway. |
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