世界中医药

目的：观察化滞柔肝颗粒对高脂诱导的非酒精性脂肪性肝炎小鼠的保护作用及初步机制。方法：将小鼠随机分成正常组、安慰剂组、低、高剂量化滞柔肝组和易善复组，通过高脂饮食喂养8周，经HE染色确定建立了非酒精性脂肪肝肝炎小鼠模型，后各组给予不同药物灌胃，正常组及安慰剂组予以生理盐水灌胃，治疗持续4周。在末次给药后，解剖动物，取血清和肝组织，进行相应的检查。结果：在治疗4周末，HE染色及油红显示模型组肝组织细胞肿胀，脂肪沉积，其体质量、血清ALT、TG水平分别为(41.2±1.7)g、（81.07±3.19）U/L和（2.69±1.30）mmol/L，均显著高于正常组的体质量、血清ALT、TG水平（21.31±1.42）g、（31.2±2.74）U/L和（0.48±0.14）mmol/L（P<0.05），结果显示模型成功建立；后续HE染色、油红染色显示高剂量化滞柔肝组改善肝脏炎性反应及脂质沉积的作用较安慰剂组、低剂量化滞柔肝颗粒组、易善复组更加明显，且后续血清ALT及TG水平结果也和上述结果一致（P<0.05）,但各观察组之间AST水平均无明显变化；通过对血清中细胞因子IL-18和IL-1β的检测，结果显示化滞柔肝颗粒组可降低细胞因子IL-18和IL-1β的水平，降低细胞因子的水平且较安慰剂组、易善复组更为明显，且呈剂量依耐性（P<0.05）。结论：化滞柔肝颗粒对高脂饮食诱导的非酒精性脂肪肝肝炎小鼠有保护作用，且和细胞因子IL-18和IL-1β有一定的相关性。

English Summary:

To observe the protective effect of Modified Huazhi Rougan Granule on mice model with nonalcoholic steatohepatitis induced by high fat diet and explore its preliminary mechanism.Methods:The mice were randomly divided into normal group,placebo group,low and high dose of Huazhi Rougan Granule group and YiShanFu group.The mice were fed with high-fat diet for 8 weeks,then these mice were confirmed by HE staining to establish the model of nonalcoholic steatohepatitis.After that,each group was given different drugs by gavage for 4 weeks.At the same time,the normal group and placebo group were given normal saline by gavage for 4 weeks.After the last administration,the animals were dissected,serum and liver tissues were taken for corresponding examination.Results:At the end of the 4th week of treatment,HE and oil red staining showed that liver cells were swollen and filled by fat deposition in the model group,and the body weight,serum ALT and TG levels were(41.2±1.7)g,(81.07±3.19)U/L and(2.69±1.30)mmol/L respectively,which were significantly higher than those in the normal group(21.31±1.42)g,(31.2±2.74)U/L and(0.48±0.14)mmol/L(P<0.05); following HE and oil red staining showed that the effect of high dose of Huazhi Rougan Granule group on improving liver inflammation and lipid deposition was more obvious than that of placebo group,low dose of Huazhi Rougan Granule group and Yishanfu group,and the follow-up serum ALT and TG levels were consistent with the above results(P<0.05).However,there was no significant change in AST level among the experimental groups; through the detection of cytokines IL-18 and IL-1 β in serum,the results showed that the Huazhi Rougan Granule group could reduce the levels of cytokines IL-18 and IL-1 β,and reduce the level of cytokines,which was more remarkable than other groups(P<0.05).Conclusion:Huazhi Rougan Granule has protective effect on mice model with nonalcoholic steatohepatitis induced by high-fat diet,and it has certain correlation with IL-18 and IL-1 β.

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