| 引用本文:赵怡然1，付洪涛1，赵楠楠2.丹参素对IUGR胎鼠脑神经细胞及脑组织Rho/ROCK信号通路的影响[J].世界中医药,2020,(22):. |
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| 丹参素对IUGR胎鼠脑神经细胞及脑组织Rho/ROCK信号通路的影响 |
| Effects of Tanshinolon on Rho/ROCK Signaling Pathway in Brain Neurons and Brain Tissue of IUGR Fetal Rats |
| 投稿时间：2020-09-22 |
| DOI：10.3969/j.issn.1673-7202.2020.22.007 |
| 中文关键词: 丹参素 宫内生长受限 神经干细胞 Ras同源基因A Rho相关螺旋卷曲蛋白激酶Ⅱ |
| English Keywords:Tanshinol Intrauterine growth restriction Neural stem cells Ras homologous gene A Rho related helix protein kinase Ⅱ |
| 基金项目:河北省2018年度医学科学研究重点课题计划（20181319） |
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| 中文摘要: |
| 目的：探讨丹参素对IUGR胎鼠脑神经细胞及脑组织Rho/ROCK信号通路的影响。方法：将40只孕鼠以随机数字表法分为正常组、IUGR组、IUGR+低剂量、中剂量、高剂量丹参素组，予以低蛋白饮食法建立IUGR模型，正常组、IUGR组于妊娠第12天尾静脉注射等体积生理盐水5.0 mg/（kg·d），丹参素低、中、高剂量组分别于妊娠第12天起尾静脉注射等体积不同浓度丹参素溶液，低剂量组10 μg/（kg·d），中剂量组20 μg/（kg·d），高剂量组30 μg/（kg·d），于自然分娩后6 h内测定胎鼠体质量，麻醉处死后行脑组织HE染色，选择RT-PCR、Western blot法对RhoA、ROCKⅡmRNA及蛋白表达进行测定，流式细胞术检测脑神经细胞凋亡率。结果：IUGR组胎鼠体质量明显低于正常组，IUGR+丹参素高剂量组胎鼠体质量高于中剂量组、低剂量组（P<0.05）。IUGR组RhoA、ROCKⅡ mRNA、蛋白表达及脑神经细胞凋亡率均高于正常组，而IUGR+丹参素高剂量组低于中剂量组、低剂量组（P<0.05）。结论：丹参素可调控Rho/ROCK信号通路并抑制其活性，以促进IUGR胎鼠脑神经细胞增殖，改善其生长发育。 |
| English Summary: |
| To explore the effects of tanshinolon on Rho/ROCK signaling pathway in brain nerve cells and brain tissues of IUGR fetal rats. Methods:A total of 40 pregnant rats were randomly divided into a normal group, a IUGR group, a IUGR+ low-dose, a medium-dose and a high-dose tanshinol group. The IUGR model was established by low-protein diet. Normal group and IUGR group were injected with 5.0 mg/kg saline intravenously on the 12th day of pregnancy. Low, medium and high dose groups of tanshinolon were injected with tanshinol solution with equal volume and different concentration intravenously from the 12th day of pregnancy, respectively, with low dose group 10 μ g/(kg·d), medium dose group 20 μ g/(kg·d) and high dose group 30 μ g/(kg·d). The weight of fetal rats was measured within 6 h after natural delivery. After anesthesia, the brain tissue was stained with HE. The expressions of RhoA and ROCKⅡmRNA and protein were determined by RT-PCR and Western blot, and the apoptosis rate of brain nerve cells was detected by flow cytometry. Results:The body weight of fetal rats in IUGR group was significantly lower than that in normal group. The body weight of fetal rats in IUGR+tanshinolhigh-dose group was higher than that in medium dose group and low-dose group (P<0.05). The expression of RhoA, rock Ⅱ mRNA, protein and apoptosis rate of brain nerve cells in IUGR group were higher than those in normal group, while those in IUGR+tanshinol high-dose group were lower than those in middle dose group and low-dose group (P<0.05). Conclusion:Tanshinol can regulate Rho/ROCK signaling pathway and inhibit its activity, so as to promote the proliferation and improve the growth and development of IUGR fetal rat brain cells. |
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