| 引用本文:韩鹏勃1，刘柱1，姚亚波1，蒋锋2.加味身痛逐瘀汤对骨质疏松模型大鼠生物力学及形态学的影响[J].世界中医药,2020,(22):. |
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| 加味身痛逐瘀汤对骨质疏松模型大鼠生物力学及形态学的影响 |
| Effect of Jiawei Shentong Zhuyu Decoction on Biomechanics and Morphology of Osteoporosis Model Rats |
| 投稿时间：2019-10-16 |
| DOI：10.3969/j.issn.1673-7202.2020.22.010 |
| 中文关键词: 骨质疏松 大鼠 加味身痛逐瘀汤 生物力学 形态学 HE染色 金属微量元素 |
| English Keywords:Osteoporosis Rat Jiawei Shentong Zhuyu Decoction Biomechanics Morphology HE staining Metal trace elements |
| 基金项目:陕西省自然科学基础研究计划项目（2017JM8146） |
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| 中文摘要: |
| 目的：探讨加味身痛逐瘀汤对骨质疏松模型鼠行为学的影响，同时观察大鼠骨组织的形态学改变。方法：45只雌性SD(Sprague Dawley）大鼠，随机分为空白组、模型组及中药组，每组15只。其中模型组和中药组进行双侧卵巢切除术复制骨质疏松模型，随后中药组接受加味身痛逐瘀汤灌胃，空白组和模型组大鼠接受等剂量生理盐水灌胃，1次/d，5次/周，连续灌胃5周。干预接受后进行生物力学检测和观察骨形态学变化，同时检测各组大鼠外周血微量元素（Fe、Zn、Mg）水平变化。结果：与空白组比较，模型组大鼠股骨最大载荷、断裂载荷、弹性载荷、刚性系数、弯曲能量降低，差异有统计学意义（P<0.05），组织形态学显示骨小梁数量、骨小梁厚度和骨小梁面积百分比均明显下降（P<0.05）；加味身痛逐瘀汤灌胃5周后中药组大鼠的股骨最大载荷、断裂载荷、弹性载荷、刚性系数、弯曲能量较模型组升高，差异有统计学意义（P<0.05），且骨小梁厚度、骨小梁数量等形态学参数也均出现明显改善，差异有统计学意义（P<0.05）。ELISA检测显示模型组大鼠外周血清Fe、Zn、Mg水平均显著下降，差异有统计学意义（P<0.05）。加味身痛逐瘀汤灌胃5周后大鼠外周血清Fe、Zn、Mg水平有所升高，与模型组比较差异有统计学意义（P<0.05），结论：加味身痛逐瘀汤对骨质疏松模型大鼠骨组织显微结构及生物力学性能均有明显改善效果，这可能通过调节机体金属元素表达有关，可推广应用。 |
| English Summary: |
| To investigate the effect of Jiawei Shentong Zhuyu decoction on the behavior of osteoporotic model rats and observe the morphological changes of bone tissue in rats.Methods:A total of 45 female SD (Sprague Dawley) rats were randomly divided into blank group (n=15),model group (n=15) and traditional Chinese medicine group (n=15).The model group and traditional Chinese medicine group received bilateral ovariectomy to establish the model of osteoporosis,then the traditional Chinese medicine group received Jiawei Shentong Zhuyu decoction,and the rats in the blank group and model group received the same dose of normal saline once a day and 5 times a week for 5 weeks.After intervention,biomechanical examination and morphological changes of bone were observed.At the same time,the levels of trace elements (Fe,Zn,Mg) in peripheral blood of rats in each group were detected.Results:Compared with the blank group,the maximum load,fracture load,elastic load,rigidity coefficient and bending energy of femur in the model group were significantly lower (P<0.05).Histomorphology showed that the number,thickness and percentage of trabecular area of bone trabeculae decreased significantly (P<0.05).After oral administration of Jiawei Shentongzhuyu decoction for 5 weeks,the maximum load,fracture load,elastic load,rigidity coefficient and bending energy of femur in the traditional Chinese medicine group were significantly higher than those in the model group (P<0.05).The morphological parameters such as trabecular thickness and number of trabeculae were also significantly improved,the difference was statistically significant (P<0.05).ELISA detection showed that the levels of Fe,Zn and Mg in peripheral serum of rats in the model group decreased significantly,and the difference was statistically significant（P<0.05）.After 5 weeks of oral administration of Jiawei Shentong Zhuyu decoction,the levels of Fe,Zn and Mg in peripheral serum of rats were significantly higher than those of the model group（P<0.05）.Conclusion:Jiawei Shentong Zhuyu decoction can significantly improve the microstructure and biomechanical properties of bone tissue of osteoporotic rats,which may be related to regulating the expression of metal elements in the body and can be popularized and applied. |
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