世界中医药

目的:以活血降糖饮干预糖尿病心肌病动物模型,从线粒体自噬角度分析活血降糖饮对糖尿病心肌病的作用机制。方法:雄性Wistar大鼠用随机区组设计分为正常组、模型组、罗格列酮组和活血降糖饮组,后3组采用STZ诱导糖尿病大鼠模型。活血降糖饮组给予2.96 g/(kg·d)灌胃处理,罗格列酮组给予4 mg/(kg·d)灌胃处理,给药8周结束后分别测定总胆固醇、三酰甘油、肌酸激酶、乳酸脱氢酶,并进行超声心动图检测,采集心肌组织,流式细胞术检测线粒体膜电位,Western-blot检测Pink1、Parkin、Mfn1、Mfn2、Beclin1的蛋白水平。结果:与正常组比较,模型组CK、LDH、TC、TG水平均显著升高,心功能下降,膜电位变化比例明显升高,Pink1、Parkin、Mfn1、Mfn2、Beclin1蛋白表达水平呈下降趋势;活血降糖饮组和罗格列酮组处理后,CK、LDH、TC、TG水平均下降,心功能得到改善,膜电位变化比例下降,Pink1、Parkin、Mfn1、Mfn2、Beclin1蛋白表达水平显著升高。结论:活血降糖饮可以显著降低糖尿病心肌病大鼠CK、LDH、TC、TG水平,改善糖尿病心肌病大鼠心功能,并且进一步发现其可以通过上调Pink1、Parkin、Mfn1、Mfn2、Beclin1表达,促进线粒体自噬,保护心肌细胞。

English Summary:

To analyze the mechanism of Huoxue Jiangtang Drink on diabetic cardiomyopathy from the perspective of mitochondrial autophagy based on the animal models of diabetic cardiomyopathy intervened by Huoxue Jiangtang Drink.Methods:Male Wistar rats were randomly divided into a normal group,a model group,a rosiglitazone group and a Huoxue Jiangtang Drink group.The last 3 groups used STZ-induced diabetic rat models.Huoxue Jiangtang Drink group was treated with 2.96 g/(kg·d) intragastric administration,and the rosiglitazone group was treated with 4 mg/(kg·d) intragastric administration.After 8 weeks,total cholesterol,triglyceride,creatine kinase and lactate dehydrogenase were tested,echocardiography was conducted,myocardial tissue was collected,mitochondrial membrane potential was detected by flow cytometry.Myocardial tissue was collected,mitochondrial membrane potential was detected by flow cytometry,and the protein levels of Pink1,Parkin,Mfn1,Mfn2,and Beclin1 were detected by Western-blot.Results:Compared with the normal group,the model group had a significant increase in CK,LDH,TC,TG,a decrease in cardiac function,a remarkable increase in the ratio of membrane potential change,and a downtrend of the protein level of Pink1、Parkin、Mfn1、Mfn2、Beclin1.However,the Huoxue Jiangtang Drink group and the rosiglitazone group got a decrease in CK,LDH,TC,TG,an improvement of cardiac function,a decrease in the ratio of membrane potential changem,and a notable increase in the protein level of Pink1,Parkin,Mfn1,Mfn2,Beclin1.Conclusion:Huoxue Jiangtang Drink can significantly reduce the levels of CK,LDH,TC,TG and improve the cardiac function of diabetic cardiomyopathy rats.Futhermore,it can promote mitochondrial autophagy and protect myocardial cells by up-regulating the expression of PINK1,Parkin,Mfn1,Mfn2 and Beclin 1.

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