To analyze the effect of Astragaloside IV(AS-IV)on HepG2 cells and its mechanism.Methods:HepG2 cells were intervened with different concentrations of AS-IV(25,50,100 nmol/L).The invasion and migration of HepG2 cells were analyzed by Trans-well,wound healing and immunofluorescence assay.Western blot was used to detect the expression of Caspase-3,Caspase-9,VEGF,MMP-14,E-cadherin,N-cadherin,Wnt,β-Catenin and TCF-4.Results:CCK-8 assay showed that AS-IV-could inhibit the growth of HepG2 cells and promote apoptosis.Wound healing and trans-well showed that AS-IV could significantly inhibit the invasion and migration of HepG2 cells.Western blotting showed that VEGF and MMP-14 protein levels,N-cadherin and vimentin expression decreased,Caspase-3 and Caspase-9 expression increased after AS-IV intervention in HepG2 cells(P<0.05).At the same time,AS-IV can inhibit the expression of Wnt,β-Catenin and TCF-4 protein in HepG2 cells.Conclusion:Astragaloside IV affects the biological process of HepG-2 cells by regulating Wnt/β-Catenin pathway.