| 引用本文:白云绮1，李慧2，宋珂1，王淑琪2，高照1，孙克寒2，金秋硕1，娄利霞1，吴爱明1，吴圣贤1，聂波1，2.ApoE-/-小鼠动脉粥样硬化模型的中医证型[J].世界中医药,2021,(01):. |
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| ApoE-/-小鼠动脉粥样硬化模型的中医证型 |
| Preliminary Study on TCM Syndromes of ApoE-/-Mouse Atherosclerosis Model |
| 投稿时间：2020-02-13 |
| DOI：10.3969/j.issn.1673-7202.2021.01.011 |
| 中文关键词: 动脉粥样硬化 ApoE-/-小鼠 病证结合动物模型 痰湿血瘀毒损 四妙勇安汤 |
| English Keywords:Atherosclerosis ApoE-/-mice Disease combined syndrome animal model Phlegm dampness blood stasis toxin damage Simiao Yongan Decoction |
| 基金项目:国家科技重大专项(2017ZX09304019);国家自然科学基金青年基金项目(81403365);国家自然科学基金面上项目(81874446) |
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| 中文摘要: |
| 目的：探讨长期高脂饲养ApoE-/-小鼠中医的证型，为抗动脉粥样硬化证候中药的研究提供证型动物模型。方法：将20只雄性ApoE-/-小鼠按随机数字表法分为四妙勇安汤组（SM组）和模型组（M组），每组10只，采用高脂饲料喂养14周建立动脉粥样硬化模型，SM组于造模开始进行预防性给药。同时以C57BL/6小鼠10只作为正常对照组（N组）。检测血清总胆固醇（TC）、三酰甘油（TG）、高密度脂蛋白胆固醇（HDL-C）、低密度脂蛋白胆固醇（LDL-C）水平，观察主动脉和肝脏病理，评价是否为血瘀痰湿证型；通过IL-6、核因子-κB免疫组织化学变化评价是否为毒损证型。结果：与N组比较，M组小鼠血清TC、TG、LDL-C显著提高，HDL-C显著降低，差异有统计学意义(P<0.05)；肝脏病理出现球形脂滴及炎性细胞浸润；主动脉出现明显斑块，血管内膜厚度（IT）、中膜厚度（MT）、斑块面积（PA）及IT/MT明显增大，差异有统计学意义(P<0.05)，血管管腔面积（LA）明显减小，差异有统计学意义(P<0.05)；免疫组织化学显示IL-6、核因子-κB在主动脉的表达明显增加，差异有统计学意义(P<0.05)。与模型组比较，SM组能够明显降低ApoE-/-小鼠主动脉PA、IT及IT/MT，明显降低IL-6及核因子-κB表达，差异有统计学意义(P<0.05)；减轻肝脏病变；但对血脂水平无明显作用。结论：长期高脂饲料饲养ApoE-/-小鼠制备的动脉粥样硬化模型为痰湿血瘀毒损型病证结合动物模型。 |
| English Summary: |
| To explore the long-term high-fat feeding of ApoE-/-mice with TCM syndrome types，and provide animal models for the research of anti-atherosclerosis syndromes in traditional Chinese medicine.Methods:A total of 20 male ApoE-/-mice were divided into Simiao Yongan Decoction group (SM group) and model group (M group) according to the random number table method.10 mice in each group were fed with high-fat diet for 14 weeks to establish atherosclerosis model.Meanwhile，the SM group started preventive administration at the time of modeling.At the same time，10 mice of C57BL / 6 were used as the normal control group (group N).By measuring serum total cholesterol (TC)，triglyceride (TG)，high-density lipoprotein cholesterol (HDL-C)，and low-density lipoprotein cholesterol (LDL-C) levels，observing aorta and liver pathology to evaluate whether it was the blood stasis phlegm-dampness syndrome type; evaluating whether it was a poisonous syndrome type by the changes of IL-6 and NF-κB immunohistochemical.Results:Compared with the control group，the serum levels of TC，TG，and LDL-C in the model group were significantly increased (P<0.01)，HDL-C was significantly reduced (P<0.01); spherical lipid droplets and inflammatory cell infiltration appeared in the liver pathology; obvious plaques appeared in the aorta; aortic intima thickness (IT)，media thickness (MT)，plaque area (PA)，and IT / MT significantly increased (P<0.01)，and the vascular lumen area (LA) decreased significantly (P<0.01); immunohistochemistry showed that the expression of IL-6 and NF-κB in the aorta was significantly increased (P<0.05).Compared with the model group，the SM group can significantly reduce the aorta PA，IT and IT / MT of ApoE-/-mice (P<0.05)，and significantly reduce the expression of IL-6 and NF-κB (P<0.05); it also reduced liver disease but had no significant effect on blood lipid levels.Conclusion:The atherosclerosis model prepared by the classic long-term high-fat feed ApoE-/-mice was preliminary evaluated as an animal model of phlegm dampness and blood stasis and toxin damage. |
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