| 引用本文:王雪蕊，郭玉红，徐霄龙，白云静，刘清泉.针刺对高血压大鼠延髓头端腹外侧区氧化应激水平影响的研究[J].世界中医药,2021,(01):. |
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| 针刺对高血压大鼠延髓头端腹外侧区氧化应激水平影响的研究 |
| Effects of Acupuncture on Oxidative Stress in the Rostral Ventrolateral Medulla of Spontaneously Hypertensive Rats |
| 投稿时间：2019-10-16 |
| DOI：10.3969/j.issn.1673-7202.2021.01.012 |
| 中文关键词: 高血压 针刺 氧化应激 延髓头端腹外侧区 大鼠 |
| English Keywords:Hypertension Acupuncture Oxidative stress Rostral ventrolateral medulla Rats |
| 基金项目:国家自然科学基金青年项目（82004292）；国家自然科学基金面上项目（81973608）；国家自然科学基金专项项目（62041701）；国家科技重大专项(2017XZ10305501)；科技部公共安全风险防控与应急技术装备专项 |
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| 中文摘要: |
| 目的：从自由基来源和清除的角度探讨针刺对自发性高血压大鼠（SHR）延髓头端腹外侧区（RVLM）中氧化应激水平影响的潜在机制。方法：15只Wistar（WKY）大鼠为正常组，45只SHR大鼠随机分为高血压组、针刺组和非穴组。针刺组取“太冲穴”直刺，非穴组取非穴位浅刺，治疗1次/d，共14 d。采用遥测技术检测针刺对血压的影响；采用DHE染色检测针刺对RVLM中活性氧族（ROS）变化的影响；采用化学发光法检测针刺对RVLM中超氧化物歧化酶（SOD）、一氧化氮合酶（NOS），和NADPH氧化酶活性的影响；采用Western Blot检测针刺对RVLM中NADPH氧化酶、SOD、NOS各亚基或亚型蛋白表达的影响。结果：与正常组比较，高血压组大鼠收缩压显著增高（P<0.05），RVLM中ROS产生增多（P<0.05），NADPH氧化酶活性增高（P<0.05），其亚基gp91phox、p22phox和nNOS蛋白表达水平显著升高（P<0.05）。与高血压组和非穴组比较，针刺干预可显著下调SHR大鼠的收缩压（P<0.05），降低RVLM中ROS的水平（P<0.05），下调NADPH氧化酶活性（P<0.05）。各组中SOD和NOS活性，SOD1、SOD2和iNOS蛋白表达水平无显著改变。结论：针刺可显著改善SHR大鼠血压，降低RVLM中ROS的水平，其机制可能与下调NADPH氧化酶亚基gp91phox、p22phox、及NOS亚型nNOS的表达水平相关。 |
| English Summary: |
| To explore the potential mechanism of acupuncture on oxidative stress level in the rostral ventrolateral medulla (RVLM) of spontaneously hypertensive rats (SHR) from the perspective of free radical source and elimination.Methods:A total of 15 Wistar (WKY) rats were in the normal group，and 45 SHR rats were randomly divided into a model group，an acupuncture group and a non-acupoint group.In the acupuncture group，“Taichong” acupoints were used for treatment once a day for 14 days.In the non-acupoint group received superficial needling was used.The effects of acupuncture on blood pressure were measured by telemetry; the effects of acupuncture on the changes of reactive oxygen species (ROS) in RVLM were detected by DHE staining; the effects of acupuncture on the activities of superoxide dismutase (SOD)，nitrogen synthase (NOS) and NADPH oxidase activity in RVLM were detected by chemiluminescence; and the effects of acupuncture on the subunits or subunits expression of NADPH oxidase，SOD and NOS in RVLM were detected by Western Blot.Results:Compared with the normal group，the systolic blood pressure of the model group was significantly increased (P<0.05)，ROS production in RVLM increased (P<0.05)，the NADPH oxidase activity increased (P<0.05)，and the protein expression levels of gp91phox，p22phox and nNOS were significantly increased (P<0.05).Compared with the model group and non-acupoint group，acupuncture intervention could significantly down-regulate the systolic blood pressure of SHR rats (P<0.05)，the level of ROS in RVLM (P<0.05)，and the activity of NADPH oxidase (P<0.05).There was no significant change in the SOD and NOS activities，the protein expression of SOD1，SOD2 and iNOS among each groups.Conclusion:Acupuncture could significantly reduce high blood pressure and ROS levels in the RVLM of SHR rats.The mechanism may be related to the down-regulation of the expression of NADPH oxidase subunits gp91phox，p22phox and NOS subtype nNOS. |
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