| 引用本文:胡佳，沈波.止嗽散对咳嗽变异性哮喘大鼠气道炎性反应及外周血Th1/Th2平衡的影响[J].世界中医药,2021,(02):. |
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| 止嗽散对咳嗽变异性哮喘大鼠气道炎性反应及外周血Th1/Th2平衡的影响 |
| Effects of Zhisou Powder on Airway Inflammation and Th1/Th2 Balance in Peripheral Blood of Rats with Cough Variant Asthma |
| 投稿时间：2019-09-16 |
| DOI：10.3969/j.issn.1673-7202.2021.02.009 |
| 中文关键词: 止嗽散 咳嗽变异性哮喘 白细胞介素4 γ-干扰素 气道炎性反应 免疫平衡 |
| English Keywords:Zhisou Powder Cough variant asthma IL-4 IFN-γ Airway inflammation Immunologic balance |
| 基金项目:国家自然科学基金项目(81473609);江苏省中医药局科技项目(YB2015070) |
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| 中文摘要: |
| 目的:探讨止嗽散对咳嗽变异性哮喘大鼠气道炎性反应及外周血Th1/Th2平衡的影响。方法:48只雄性SD大鼠随机分为正常对照组、模型对照组、地塞米松组(0.2 mg/kg)、止嗽散高(12.6 g/kg)、中(6.3 g/kg)、低(3.1 g/kg)剂量组,每组8只。以卵白蛋白(OVA)致敏激发法制备大鼠咳嗽变异性哮喘模型,检测各组大鼠血液中嗜酸性粒细胞、中性粒细胞及淋巴细胞含量,采用酶联免疫吸附剂测定法(ELISA)测定血清和肺泡灌洗液(BALF)中IL-4和IFN-γ水平,同时做肺脏的组织病理学检测。结果:止嗽散高剂量组大鼠血液嗜酸性粒细胞绝对值、淋巴细胞绝对值降低,血清和BALF中IL-4含量降低,IFN-γ含量升高,与模型组比较差异有统计学意义(P<0.01,P<0.05);同时能够改善肺脏组织炎性细胞浸润,减轻纤维结缔组织增生。结论:止嗽散可以通过降低血清和BALF中IL-4含量,升高IFN-γ含量,调节Th1/Th2的免疫失衡,改善咳嗽变异性哮喘症状。 |
| English Summary: |
| To explore the effects of Zhisou Powder on airway inflammation and Th1/Th2 balance in peripheral blood of rats with cough variant asthma.Methods:A total of 48 male SD rats were randomly divided into a normal control group，a model control group，a dexamethasone group(0.2 mg/kg)，a Zhisou Powder high-dose(12.6 g/kg)，a medium-dose(6.3g/kg)and low-dose(3.1 g/kg)groups，with 8 rats in each group.Sensitized with ovalbumin(OVA)excitation of cough variant asthma model rats，each rat blood eosinophils and neutrophils and lymphocytes in content was detected.Enzyme-linked immune sorbent assay(ELISA)was used to determine serum and alveolar lavage fluid(BALF)of IL-4 and IFN-gamma level.At the same time lung tissue pathology testing was done.Results:The absolute value of eosinophil and lymphocyte in blood of rats，the content of IL-4 and IFN-γ in serum and BALF in Zhisou Powder high-dose group were decreased，and the differences were significant compared with model group(P<0.01,P<0.05).At the same time，it can improve inflammatory cell infiltration of lung tissue and reduce fibrous connective tissue hyperplasia.Conclusion:Zhisou Powder can reduce the content of IL-4 in serum and BALF，increase the content of IFN-γ，regulate the immune imbalance of Th1/Th2 and improve the symptoms of cough variant asthma. |
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