| 引用本文:南凌杉1，张雅月2，王佳3，丁晓庆1，郭明1，谌海燕1，刘军霞1，李玲1，陈信义2，郎海燕1.基于线粒体功能特性研究健脾益气摄血方调控ITP乏力机制[J].世界中医药,2021,(03):. |
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| 基于线粒体功能特性研究健脾益气摄血方调控ITP乏力机制 |
| Study on the Mechanism of Jianpi Yiqi Shexue Prescription on Regulating ITP Related Fatigue Based on Mitochondrial Functional Characteristics |
| 投稿时间：2021-01-05 |
| DOI：10.3969/j.issn.1673-7202.2021.03.002 |
| 中文关键词: 免疫性血小板减少症 乏力 健脾益气摄血 线粒体 线粒体功能 活性氧含量 线粒体DNA ATP含量 |
| English Keywords:Immune thrombocytopenia Fatigue Jianpi Yiqi Shexue Mitochondrial Mitochondrial function ROS content Mitochondrial DNA ATP content |
| 基金项目:国家自然科学基金项目（81803904，81703903） |
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| 中文摘要: |
| 目的：验证健脾益气摄血方缓解ITP乏力症状与改善线粒体功能的相关性。方法：通过被动免疫造模法建立ITP小鼠模型，分为正常组、模型组、强的松组、健脾益气摄血（JPYQSX）中、高剂量组。通过光谱法检测小鼠脾脏组织活性氧含量；通过实时荧光定量PCR（qPCR）检测线粒体DNA相对拷贝数；通过免疫荧光法检测结肠ATP含量。结果：与正常组比较，模型组（P<0.01）、强的松组（P<0.05）、健脾益气摄血各剂量组（P<0.01）小鼠脾脏组织ROS含量增多，脾脏组织线粒体DNA相对拷贝数明显下调（P<0.01），结肠组织ATP含量明显下调（P<0.01）；与模型组比较，强的松组、健脾益气摄血各剂量组小鼠脾脏组织ROS含量减少，脾脏组织线粒体DNA相对拷贝数比较无统计学意义（P>0.05），结肠组织ATP含量均显著升高（P<0.01）。结论：健脾益气摄血方可通过改善线粒体功能来有效缓解ITP乏力症状。 |
| English Summary: |
| To verify the correlation of the Jianpi Yiqi Shexue Formula in relieving ITP related fatigue and improving mitochondrial function.Methods:ITP mice model was established by the passive immunization mode method and divided into a normal group,a model group,a prednisone group,a Jianpi Yiqi Shexue Formula of medium and high dose group.The reactive oxygen content in mouse spleen tissue was detected by spectroscopy; the relative copy number of mitochondrial DNA was detected by real-time fluorescent quantitative PCR (qPCR); the ATP content in colon was detected by immunofluorescence.Results:Compared with the normal group,the content of ROS in spleen tissue of mice increased,the relative copy number of mitochondrial DNA in spleen tissue was significantly down-regulated (P<0.01),and the content of ATP in colon tissue was significantly down-regulated (P<0.01) in the model group (P<0.01),in the prednisone group (P<0.05),the Jianpi Yiqi Shexue Formula of each dose group (P<0.01); compared with the model group,the content of ROS in spleen tissue of mice in the prednisone group (P<0.05),and the Jianpi Yiqi Shexue Formula of each dose group decreased,and the relative copy number of mitochondrial DNA in spleen tissue showed no statistical difference.Conclusion:Jianpi Yiqi Shexue Formula can effectively relieve ITP related fatigue by improving mitochondrial function. |
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