To verify the correlation of the Jianpi Yiqi Shexue Formula in relieving ITP related fatigue and improving mitochondrial function.Methods:ITP mice model was established by the passive immunization mode method and divided into a normal group,a model group,a prednisone group,a Jianpi Yiqi Shexue Formula of medium and high dose group.The reactive oxygen content in mouse spleen tissue was detected by spectroscopy; the relative copy number of mitochondrial DNA was detected by real-time fluorescent quantitative PCR (qPCR); the ATP content in colon was detected by immunofluorescence.Results:Compared with the normal group,the content of ROS in spleen tissue of mice increased,the relative copy number of mitochondrial DNA in spleen tissue was significantly down-regulated (P<0.01),and the content of ATP in colon tissue was significantly down-regulated (P<0.01) in the model group (P<0.01),in the prednisone group (P<0.05),the Jianpi Yiqi Shexue Formula of each dose group (P<0.01); compared with the model group,the content of ROS in spleen tissue of mice in the prednisone group (P<0.05),and the Jianpi Yiqi Shexue Formula of each dose group decreased,and the relative copy number of mitochondrial DNA in spleen tissue showed no statistical difference.Conclusion:Jianpi Yiqi Shexue Formula can effectively relieve ITP related fatigue by improving mitochondrial function.