To explore the ITP hemostatic mechanism of “treating from spleen” therapy based on the characteristics of vasoactive factors.Methods:The ITP mouse model was established by passive immunomodeling method.The prednisone,Jianpi Yiqi Formula,Jianpi Yiqi Shexue Formula and Guipi Decoction were used as intervention drugs.The content of serum ET-1,NO,NOS3,TXA2,PGI2,VWF,VCAM-1 and TM of the model mice were detected by enzyme-linked immunosorbent assay (ELISA).Results:1）Therapeutic effect of platelet enhancement:compared with normal control group,PLT count in peripheral blood of mice in each group was significantly decreased after APS injection (P<0.01); compared with model control group,PLT count in peripheral blood of mice in each group was significantly increased on the 8th day of injection (P<0.01).2）Hemostasis effect:except for the normal control group,all experimental groups had bleeding tendency to a greater or lesser extent before injection; compared with model control group,the grade of bleeding in experimental groups decreased on the 6th and 8th day of administration (P<0.01).3）Vascular coagulation factor:compared with normal group,ET-1 detection value in each group was significantly decreased (P<0.01); compared with normal control group and model control group,TXA2 detection values in experimental groups were significantly increased (P<0.01).Compared with normal group,VWF detection values in experimental groups were significantly down-regulated (P<0.01).Compared with normal control group,VCAM-1 detection value in experimental groups was significantly reduced (P<0.01).4）Vascular anticoagulant factor:compared with normal group,NO detection value in experimental groups was significantly decreased (P<0.01); Compared with normal control group,NOS3 values in all groups were expressively reduced (P<0.05) except for Guipi Decoction group.Compared with normal control group,PGI2 detection value in experimental groups was significantly cut down (P<0.01).Compared with normal control group,TM detection values in experimental groups were greatly reduced (P>0.05).Conclusion:PLT count in peripheral blood of ITP model mice from the treatment of spleen theory is increased,and effectively hemostasis is taken,which has a close correlation between increasing TXA2 and VCAM-1,PGI2 and TM detection values are decreased,and balancing pro-coagulation and anticoagulation factors.