| 引用本文:薛艳1，许嘉慧2，高艳荣1，陈飞飞1，孙仕奇1，张炜1.基于网络药理学探讨清肺化痰方改善慢性阻塞性肺疾病的作用机制[J].世界中医药,2021,(04):. |
|
| 基于网络药理学探讨清肺化痰方改善慢性阻塞性肺疾病的作用机制 |
| Exploring the Mechanism of Therapeutic Effect of Qingfei Huatan Decoction on Chronic Obstructive Pulmonary Disease Based on Network Pharmacology |
| 投稿时间：2020-07-21 |
| DOI：10.3969/j.issn.1673-7202.2021.04.006 |
| 中文关键词: 网络药理学 分子对接 气道黏液高分泌 慢性阻塞性肺疾病急性加重期 清肺化痰方 |
| English Keywords:Network pharmacology Molecular docking Airway mucus hypersecretion Acute exacerbation of chronic obstructive pulmonary disease(AECOPD) Qingfei Huatan Decoction |
| 基金项目:上海市科委上海中医临床重点实验室项目(14DZ2273200)；上海市临床重点专科(中医肺病科)项目（shslczdzk05101） |
|
| 摘要点击次数: 881 |
| 全文下载次数: 0 |
| 中文摘要: |
| 目的：通过网络药理学及分子对接技术探究清肺化痰方改善慢性阻塞性肺疾病急性加重期(AECOPD)气道黏液高分泌的有效成分和潜在分子机制。方法：首先使用中药系统药理学分析平台（TCMSP）收集清肺化痰方的活性成分和潜在靶点，利用GeneGards等多个数据库筛选AECOPD气道黏液高分泌的疾病靶点；然后将疾病靶点与药物预测靶点取交集，借助Cytoscape3.7.2软件构建交集靶点网络以及蛋白质-蛋白质相互作用网络，利用DAVID进行GO和KEGG富集分析。最后，将药物活性成分与疾病靶点进行分子对接。结果:清肺化痰方中54个有效活性成分可通过67个潜在靶标改善AECOPD的气道黏液高分泌,涉及TNF信号通路、IL-17、PI3K-Akt等相关信号通路。槲皮素、木犀草苷、β-谷甾醇等成分与AKT1、IL6、EGF等靶标蛋白结合稳定。结论：初步揭示清肺化痰方可直接改善AECOPD气道黏液高分泌，以及其多靶点、多通路的潜在作用机制,为后续研究提供思路。 |
| English Summary: |
| To explore the potential active components and underlying molecular mechanisms of Qingfei Huatan Decoction to improve the hypersecretion of airway mucus in acute exacerbation of chronic obstructive pulmonary disease(AECOPD)through the network pharmacology and molecular docking technology.Methods:First，the active components and potential targets of Qingfei Huatan Decoction were collected through the traditional Chinese medicine system pharmacology analysis platform(TCMSP)，and the targets related to airway mucus hypersecretion in AECOPD were screened by GeneCards database.Then，the disease target and the drug prediction target were intersected，and the intersection target network and protein interaction network were constructed with the help of Cytoscape 3.7.2 software，and the GO and KEGG enrichment analysis were performed by DAVID.Finally，the active ingredient of the drug was molecularly docked with the disease target.Results:A total of 54 effective active ingredients of Qingfei Huatan Decoction were obtained，which could act on AECOPD airway mucus hypersecretion through 67 potential targets，involving TNF signaling pathway，IL-17，epidermal growth factor receptor tyrosine kinase inhibitors resistance，PI3K-Akt，HIF-1，MAPK and other related signaling pathways.Molecular docking results showed that quercetin，luteolin，beta-sitosterol and other pharmaceutical active ingredients could bind to AKT1，IL6，EGF and other target proteins stably.Conclusion:The molecular docking technique reveals that Qingfei Huatan Decoction can directly improve the airway mucus hypersecretion of AECOPD and achieve therapeutic effects.Through the network pharmacology，the potential mechanism of its multi-target and multi-pathway was initially revealed，which provides ideas for further research． |
| 查看全文 查看/发表评论 下载PDF阅读器 |
