To observe the effect of Lianggan Xifeng Zhichan Formula (LGXFZC) on Parkinson's disease (Parkinson's disease,PD) and explore its neuroprotective mechanism.Methods:A total of 60 SD rats were divided into a sham operation group (n=10) and a model group (n=50).The rats in the model group were made by PD model.40 rats were randomly divided into a model group (n=10),a low dose group (0.36 g/kg) (n=10),a middle dose group (0.72 g/kg) and a high dose group (1.44 g/kg) for 30 days.The rotation times of apomorphine,cylinder test,ultrastructure of striatum and the levels of CaMkk β,AMPK and p-AMPK were compared in each group.Results:1) LGXFZC could significantly reduce the frequency of rotation and limb touching wall in rats.As the dose increases,the frequency decreases more obviously.The difference was statistically significant (P<0.05).2) the phenomenon of mitochondrial autophagy in striatum of model rats was weakened,and LGXFZC could obviously promote mitochondrial autophagy in brain tissue.3) after the intervention of Chinese medicine,the expression of CaMkk β and p-AMPK protein in the striatum of rats was significantly higher than that in the model group (P<0.05),and compared with the model group,the difference is statistically significant (P<0.05).Among them,the high-dose group and the lower-dose group were up-regulated significantly (P<0.05).Conclusion:LGXFZC has neuroprotective effect,and one of its mechanisms may be that it promotes mitochondrial autophagy by activating the activity of CaMkk/AMPK pathway.