世界中医药
文章摘要
引用本文:刘珊,敬秀平,谢安卫,尹诗.天王补心丹对失眠模型鼠的疗效及部分机制研究[J].世界中医药,2021,(05):.  
天王补心丹对失眠模型鼠的疗效及部分机制研究
Study on the Therapeutic Effect and Partial Mechanism of Tianwang Buxin Pill on Insomnia Model Rats
投稿时间:2019-10-12  
DOI:10.3969/j.issn.1673-7202.2021.05.017
中文关键词:  失眠  模型大鼠  天王补心丹  行为学  中缝背核Toll样受体4信号通路  关键因子  肿瘤坏死因子-α  白细胞介素-1
English Keywords:Insomnia  Model rats  Tianwang Buxin Pill  Behavior  TLR4 signaling pathway  Key factors  TNF-α  IL-1
基金项目:国家自然科学基金项目(81273851)
作者单位
刘珊,敬秀平,谢安卫,尹诗 四川省广安市人民医院综合科广安638500 
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中文摘要:
      目的:观察天王补心丹对失眠模型鼠行为学的影响,探析其对TLR4通路的影响。方法:利用氯苯丙氨酸腹腔注射制备失眠模型,将成模45只大鼠数字随机分为模型组、天王补心丹低剂量组(7 g/kg)及天王补心丹高剂量组(28 g/kg),另设空白组15只。连续中药干预7 d后比较各组大鼠旷场行为学、睡眠时间、中缝背核Toll样受体4(TLR4)、髓样分化蛋白88(MyD88)以及外周血清核因子-κB、诱导干扰素β的TRIF变化。结果:天王补心丹可明显减少失眠模型大鼠中央格停留时间、修饰次数,延长睡眠时间缩短睡眠潜伏期,差异有统计学意义(P<0.05),外周血核因子-κB、TRIF、肿瘤坏死因子-α(TNF-α)、IL-1以及中缝背核TLR4、MyD88水平均降低,差异有统计学意义(P<0.05)。结论:天王补心丹可改善失眠,其作用机制可能与通过TLR4通路调节机体免疫有关。
English Summary:
      To observe the effect of Tianwang Buxin Pill on the behavior of insomnia model rats and to explore its effect on TLR4 pathway.Methods:The insomnia model was established by intraperitoneal injection of chlorophenylalanine.A total of 45 rats were randomly divided into a model group,a low dose Tianwang Buxin Pill group (7 g/kg) and a high dose Tianwang Buxin Pill group (28 g/kg).Another 15 rats in the blank group were established.After continuous intervention with Chinese medicine for 7 days,the changes of open field behavior,sleep time,Toll-like receptor 4 (TLR4),myeloid differentiation protein 88 (MyD88) and peripheral serum nuclear factor-kappa B (NF-kappa B),TIR base domain linker molecule for inducing interferon β (TRIF) were compared in each group.Results:Tianwang Buxin Pill could significantly reduce the central lattice residence time and modification times of insomnia model rats,and prolong the sleep time and shorten the sleep latency (P<0.05).The levels of NF-κ B,TRIF,TNF-α,IL-1 in peripheral blood and TLR4 and MyD88 in dorsal raphe nucleus decreased (P<0.05).Conclusion:Tianwang Buxin Pill can improve insomnia,and its mechanism may be related to the regulation of immunity through TLR4 pathway.
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