| 引用本文:刘珊，敬秀平，谢安卫，尹诗.天王补心丹对失眠模型鼠的疗效及部分机制研究[J].世界中医药,2021,(05):. |
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| 天王补心丹对失眠模型鼠的疗效及部分机制研究 |
| Study on the Therapeutic Effect and Partial Mechanism of Tianwang Buxin Pill on Insomnia Model Rats |
| 投稿时间：2019-10-12 |
| DOI：10.3969/j.issn.1673-7202.2021.05.017 |
| 中文关键词: 失眠 模型大鼠 天王补心丹 行为学 中缝背核Toll样受体4信号通路 关键因子 肿瘤坏死因子-α 白细胞介素-1 |
| English Keywords:Insomnia Model rats Tianwang Buxin Pill Behavior TLR4 signaling pathway Key factors TNF-α IL-1 |
| 基金项目:国家自然科学基金项目（81273851） |
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| 中文摘要: |
| 目的：观察天王补心丹对失眠模型鼠行为学的影响，探析其对TLR4通路的影响。方法：利用氯苯丙氨酸腹腔注射制备失眠模型，将成模45只大鼠数字随机分为模型组、天王补心丹低剂量组（7 g/kg）及天王补心丹高剂量组（28 g/kg），另设空白组15只。连续中药干预7 d后比较各组大鼠旷场行为学、睡眠时间、中缝背核Toll样受体4（TLR4）、髓样分化蛋白88（MyD88）以及外周血清核因子-κB、诱导干扰素β的TRIF变化。结果：天王补心丹可明显减少失眠模型大鼠中央格停留时间、修饰次数，延长睡眠时间缩短睡眠潜伏期，差异有统计学意义（P<0.05），外周血核因子-κB、TRIF、肿瘤坏死因子-α（TNF-α）、IL-1以及中缝背核TLR4、MyD88水平均降低，差异有统计学意义（P<0.05）。结论：天王补心丹可改善失眠，其作用机制可能与通过TLR4通路调节机体免疫有关。 |
| English Summary: |
| To observe the effect of Tianwang Buxin Pill on the behavior of insomnia model rats and to explore its effect on TLR4 pathway.Methods:The insomnia model was established by intraperitoneal injection of chlorophenylalanine.A total of 45 rats were randomly divided into a model group,a low dose Tianwang Buxin Pill group (7 g/kg) and a high dose Tianwang Buxin Pill group (28 g/kg).Another 15 rats in the blank group were established.After continuous intervention with Chinese medicine for 7 days,the changes of open field behavior,sleep time,Toll-like receptor 4 (TLR4),myeloid differentiation protein 88 (MyD88) and peripheral serum nuclear factor-kappa B (NF-kappa B),TIR base domain linker molecule for inducing interferon β (TRIF) were compared in each group.Results:Tianwang Buxin Pill could significantly reduce the central lattice residence time and modification times of insomnia model rats,and prolong the sleep time and shorten the sleep latency (P<0.05).The levels of NF-κ B,TRIF,TNF-α,IL-1 in peripheral blood and TLR4 and MyD88 in dorsal raphe nucleus decreased (P<0.05).Conclusion:Tianwang Buxin Pill can improve insomnia,and its mechanism may be related to the regulation of immunity through TLR4 pathway. |
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