世界中医药

目的：根据网络药理学方法对肾衰饮治疗糖尿病肾病(DKD)的作用机制进行探讨。方法：利用中药分子机制生物信息学分析工具(BATMAN-TCM)、中药系统药理学数据库与分析平台(TCMSP)对肾衰饮中有效成分进行筛选及靶点预测，检索GeneCards、TTD、DisGeNET数据库筛选DKD靶点，利用韦恩图在线平台筛选肾衰饮治疗DKD交集靶点，借助网络拓扑分析插件CytoNCA筛选五苓散治疗DKD核心靶点，使用Cytoscape 3.7.1建立化合物-疾病-靶点调控网络，基于DAVID数据库对核心靶点进行GO功能富集和KEGG信号通路富集分析。结果：基于BATMAN-TCM、TCMSP得到肾衰饮的178个有效成分和280个潜在靶点，筛选获得与DKD疾病发生、发展相关的靶点2 699个，借助网络拓扑分析插件CytoNCA筛选肾衰饮治疗DKD核心靶点31个，GO功能富集分析得到条目346个，其中生物过程条目278个、分子功能条目44个、细胞组成条目24个，主要包括氧化应激、脂质反应、生长因子受体结合、细胞因子受体结合、转录因子结合、血小板α颗粒等功能途径；KEGG信号通路富集分析得出条目101个，主要包括AGE-RAGE信号通路、TNF信号通路、IL-17信号通路、FoxO信号通路、MAPK信号通路等通路途径。结论：基于网络药理学初步探讨并验证了肾衰饮治疗DKD多成分、多靶点、多通路的整体调节作用特点，预测了肾衰饮治疗DKD的潜在作用机制，以期为其活性成分研究与实验研究提供科学依据。

English Summary:

To explore the mechanism of Shenshuaiyin(SSY)in treatment of diabetic kidney disease(DKD)based on network pharmacology.Methods:Using a bioinformatics analysis tool for molecular mechanism of traditional Chinese medicine(BATMAN-TCM)，traditional Chinese medicine systems pharmacology database and analysis platform(TCMSP)，this paper screened effective components and predicted targets of SSY，searching GeneCards，TTD，DisGeNET to screen targets of DKD and using Venn online platform to get intersection targets of SSY-DKD.By using CytoNCA common targets of SSY-DKD was selected，and then a component-disease-target network was built using Cytoscape 3.7.1.Through DAVID database GO function enrichment and KEGG pathway enrichment was analyzed.Results:A total of 178 effective components and 280 potential targets of SSY were obtained based on BATMAN-TCM and TCMSP，2699 targets related to the occurrence and development of DKD were obtained while 31 common targets of SSY-DKD were obtained by using CytoNCA.A total of 346 items were obtained by GO function enrichment analysis，namely，278 biological process items，44 molecular function items and 24 cellular component items，mainly including means of functions such as response to oxidative stress，response to lipid，growth factor receptor binding，cytokine receptor binding，transcription factor binding，platelet alpha granule.KEGG pathway enrichment analysis obtained 101 items，including AGE-RAGE signaling pathway in diabetic complications，TNF signaling pathway，IL-17 signaling pathway，FoxO signaling pathway，MAPK signaling pathway.Conclusion:Based on network pharmacology，the overall regulatory characteristics of SSY in treatment of DKD were discussed and verified，and the potential mechanism of SSY in treatment of DKD was predicted，in order to provide scientific basis for the study of active ingredients and experimental research.

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