| Dafufang Quxie Capsules are developed based on the famous prescription Yinyang Gongji Pills used by the physician LI Zhongzi in the Ming Dynasty to treat accumulation. The uses network pharmacology to explore its mechanism of action on colorectal cancer, in order to reveal the classic prescription Yin Yang Gongji Pills Provide reference for clinical value and modern mechanism. Methods:TCMSP, ETCM and other databases were searched to establish the database of active ingredients and targets of Quxie Capsules. Fishing through the Swiss Target Prediction target, we took the union of the drug targets. Through TTD, OMIM, Drugbank and GeneCards databases, we screened the targets of colorectal cancer and took the union. We took the intersection of drug target and disease target, and screened according to the degree value of the compound, established a “Chinese medicine-component-target” database, constructed a PPI network based on the core target, performed GO function and KEGG enrichment analysis, and compared the results with preliminary research. Results:The final data about Quxie Capsule for the treatment of colorectal cancer had 127 active ingredients targeting 254 core genes. The PPI network contains 248 core genes, and there were 5 subnet networks in total. Enrichment analysis results showed that Quxie Capsules may mediate HIF1α, PI3K/AKT, TNF, FoxO, Toll-like receptors through processes such as the initiation of RNA polymerase, DNA transcription, translation, protein activation and proliferation, apoptosis, drug resistance, and inflammation. And many other signaling pathways, among which the regulation of apoptosis, immunity and FoxO signaling pathway have been verified by our team's previous research. Conclusion:Mechanism of Quxie Capsules on the treatment of colorectal cancer is related to mediating apoptosis, immunity and FoxO signaling pathway. In addition, it may also regulate multiple signaling pathways such as HIF1α, PI3K/AKT, TNF, and Toll-like receptors. This revealed that Quxie Capsules have many components in the treatment of colorectal cancer, and its regulatory pathways and targets are complex. At the same time, it demonstrates the theoretical charm and clinical value of the ancients' “anti-excitement and rebellion” and “warming yang and ventilating the lower part” theory of treating tumors, providing guidance for follow-up in-depth research. |
