| 引用本文:王军，王剑，鲁敏，牧亭亭，耿亚会.异补骨脂素通过PPAR-γ-Axin2-Wnt信号通路调节对大鼠骨代谢的影响[J].世界中医药,2021,(09):. |
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| 异补骨脂素通过PPAR-γ-Axin2-Wnt信号通路调节对大鼠骨代谢的影响 |
| Effects of Isopsoralen on Bone Metabolism in Rats Via PPAR Gamma-Axin 2-Wnt Signal Regulation |
| 投稿时间：2019-06-19 |
| DOI：10.3969/j.issn.1673-7202.2021.09.011 |
| 中文关键词: 异补骨脂素 骨质疏松 骨代谢 骨组织形态 Wnt信号通路 PPAR-γ蛋白 Axin2蛋白 β-catenin蛋白 |
| English Keywords:Isopsoralen Osteoporosis Bone metabolism Bone histomorphology Wnt signaling pathway PPAR-γ protein AXIN2 protein β-Catenin protein |
| 基金项目:国家自然科学基金地区科学基金项目（81560368） |
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| 中文摘要: |
| 目的：探讨异补骨脂素通过PPAR-γ-Axin2-Wnt信号通路调节对大鼠骨代谢的影响。方法：选择雌性大鼠40只，随机分为模型组和异补骨脂素组，各20只，实验12周后，对大鼠骨密度、骨组织形态进行观察，并检测各组大鼠血清骨代谢生化指标情况，采用蛋白质免疫印迹法（Western blotting)检测PPAR-γ、Axin2及β-catenin蛋白的表达情况。结果：12周后，模型组大鼠股骨、骨盆、脊柱及全身骨密度值均低于异补骨脂素组（P<0.05）；异补骨脂素组大鼠血清钙、磷及抗酒石酸酸性磷酸酶(Tartrate Resistant Acid Phosphatase，TRACP)水平低于模型组，血清碱性磷酸酶(Alkaline Phosphatase，ALP)水平高于模型组（P<0.05）；骨组织形态计量学分析结果显示，模型组大鼠骨小梁稀疏、断裂明显，而异补骨脂素组大鼠骨小梁面积百分数和骨小梁数目明显增加，骨小梁分离度明显下降（P<0.05）。12周后，与模型组比较，异补骨脂素组PPAR-γ和Axin2蛋白水平下调，β-catenin蛋白表达明显增加（P<0.05）。结论：异补骨脂素可能通过抑制Axin2/PPAR-γ信号通路，激活Wnt信号通路，改善大鼠骨代谢。 |
| English Summary: |
| To investigate the effects of isopsoralen on bone metabolism in rats through PPAR-gamma-Axin2-Wnt signal regulation.Methods:A total of 40 female rats were randomly divided into a model group and an isopsoralen group with 20 rats in each group.After 12 weeks of experiment，the bone mineral density(BMD) and bone histomorphology of rats were observed，and the biochemical indexes of bone metabolism in serum of rats in each group were detected.The expressions of PPAR-gamma，Axin 2 and beta-catenin protein were detected by Western blotting.Results:After 12 weeks，BMD values of femur，pelvis，spine and whole body in model group were lower than those in isopsoralen group(P<0.05); serum Ca，P and TRACP levels in isopsoralen group were lower than those in model group，and serum ALP levels were higher than those in model group(P<0.05); bone histomorphometric analysis showed that the trabeculae of model group were sparse and fractured obviously.The percentage of bone trabecular area and the number of bone trabeculae increased significantly，and the degree of trabecular segregation decreased significantly(P<0.05).After 12 weeks，compared with model rats，PPAR-gamma and Axin 2 protein levels in isopsoralen group were down-regulated and beta-catenin protein expression was significantly increased(P<0.05).Conclusion:Isopsoralen may improve the abnormal bone metabolism in rats by inhibiting Axin2/PPAR gamma signaling pathway，activating Wnt signaling pathway and improving bone metabolism in rats. |
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