| 引用本文:窦金金1，齐珊2，张喜武2.加减黄连温胆汤对血管性痴呆模型大鼠炎症反应的影响[J].世界中医药,2021,(09):. |
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| 加减黄连温胆汤对血管性痴呆模型大鼠炎症反应的影响 |
| Effects of Modified Huanglian Wendan Decoction on Inflammation Reaction in Rats with Vascular Dementia |
| 投稿时间：2020-10-14 |
| DOI：10.3969/j.issn.1673-7202.2021.09.012 |
| 中文关键词: 加减黄连温胆汤 血管性痴呆 炎症反应 逆转录-聚合酶链反应 白细胞介素-1βmRNA 肿瘤坏死因子-αmRNA 环氧合酶-2 mRNA |
| English Keywords:Modified Huanglian Wendan Decoction Vascular dementia Inflammation reverse transcription-polymerase chain reaction Interleukin-1βmRNA Tumor necrosisfactor-α TNF-αmRNA Cyclooxygenase-2mRNA |
| 基金项目:国家自然科学基金面上项目（81703719）；黑龙江省博士后科研启动基金项目(LBH-Q16215)；黑龙江省自然科学基金项目（LH2019H102） |
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| 中文摘要: |
| 目的：观察加减黄连温胆汤对血管性痴呆(Vascular Dementia,VD)模型大鼠脑组织白细胞介素-1β（Interleukin-1β,IL-1β）mRNA、肿瘤坏死因子-α（Tumor Necrosis Factor-α,TNF-α）mRNA和环氧合酶-2（Cyclooxygenase-2,COX-2）mRNA的影响，明确炎症反应对VD的影响，探讨加减黄连温胆汤对VD大鼠的炎症作用机制。方法：采用大鼠双侧颈总动脉间断性结扎的方法，复制VD大鼠模型。通过逆转录-聚合酶链反应（Reverse Transcription-polymerase Chain Reaction,RT-PCR）法试剂盒检测TNF-αmRNA、IL-1βmRNA和COX-2 mRNA，并描绘各验证因子数据表达变化。结果：模型组与空白组、假手术组比较，模型组脑组织IL-1βmRNA和TNF-αmRNA及COX-2mRNA的表达明显增多，有显著性差异；加减黄连温胆汤与模型组比较，加减黄连温胆汤可使IL-1βmRNA和TNF-αmRNA及COX-2mRNA的表达下调，有显著性差异。结论：加减黄连温胆汤可通过抑制炎症介质IL-1βmRNA、TNF-αmRNA及COX-2 mRNA的表达，发挥对VD模型大鼠的治疗作用。 |
| English Summary: |
| To observe the effects of modified Huanglian Wendan Decoction on interleukin-1β(IL-1β）mRNA,tumor necrosis factor-α(TNF-α） mRNA and cyclooxygenase-2(COX-2)mRNA in the brain tissue of vascular dementia(VD）model rats.To clarify the influence of inflammation on VD，and to explore the inflammatory mechanism of modified Huanglian Wendan Decoction on VD rats.Methods:The rat model of VD was replicated by intermittent ligation of bilateral common carotid arteries in rats.TNF-αmRNA，IL-1βmRNA and COX-2 mRNA were detected by reverse transcription-polymerase chain reaction(RT-PCR）kit，and the expression changes of each verification factor data were depicted.Results:Compared with the blank group and the sham operation group，the expression of IL-1βmRNA，TNF-αmRNA and COX-2mRNA in the brain tissue of the model group was significantly increased，and there was a significant difference.Compared with the model group，modified Huanglian Wendan Decoction，can down-regulate the expression of IL-1βmRNA，TNF-αmRNA and COX-2mRNA，with significant differences.Conclusion:Modified Huanglian Wendan can reduce the inhibition of inflammatory factor the expression of IL-1βmRNA，TNF-αmRNA and COX-2mRNA，and play a role in inhibiting inflammation. |
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