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引用本文:周亚兵，蒋思韵，王利维，华丽.PM2.5对哮喘大鼠IL-17/IL-23炎症介质的影响及人参皂苷Rg1干预研究[J].世界中医药,2021,(10):.

PM2.5对哮喘大鼠IL-17/IL-23炎症介质的影响及人参皂苷Rg1干预研究

Effect of PM2.5 on IL-17/IL-23 in asthmatic rats and intervention of Ginsenoside Rg1

投稿时间：2020-09-20

DOI：10.3969/j.issn.1673-7202.2021.10.004

中文关键词: 哮喘大气颗粒物2.5 辅助性T细胞17 白细胞介素-17 白细胞介素-23 维A酸相关孤儿受体γt 人参皂苷Rg1

English Keywords:Asthma atmospheric particulate matter 2.5 helper T cell 17 IL-17 IL-23 retinoic acid related orphan receptor γt ginsenoside Rg1

基金项目:国家临床重点专科项目(ZJ0901EK013)；上海市自然科学基金项目（14140903000）；上海市科委2020年度“科技创新行动计划”医学创新研究专项（20Y21900500）；上海市十三五临床重点专科（中医专业）项目（SHSLCZDZK04103）

作者	单位
周亚兵，蒋思韵，王利维，华丽	上海交通大学医学院附属新华医院，上海，200092

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中文摘要:

目的：基于气道IL-17/IL-23炎症介质轴，探讨人参皂苷Rg1对PM2.5哮喘大鼠肺损伤保护机制。方法：采集并分离交通相关大气细颗粒物PM2.5，利用卵白蛋白致敏和激发建立幼龄哮喘大鼠模型，再给予PM2.5气管滴注建立PM2.5哮喘肺损伤模型，采用酶联免疫吸附试验（ELISA）法测定血清和肺泡灌洗液IL-17a、IL-6、IL-23、TGF-β1含量，RT-PCR检测RORγt mRNA表达，并行肺组织病理和电镜分析。结果：成功建立PM2.5气管滴注哮喘大鼠模型；哮喘大鼠模型血清和肺泡灌洗液IL-17a、IL-6、IL-23、TGF-β1表达量增大（P<0.01），肺组织RORγt mRNA表达量增加（P<0.01）；经PM2.5气管滴注后，模型组大鼠血清和肺泡灌洗液IL-17a、IL-6、IL-23、TGF-β1含量进一步增加，RORγt mRNA表达量进一步上升（P<0.01）；经皂苷Rg1干预后，呈现剂量依赖性下调血清和肺泡灌洗液IL-17a、IL-6、IL-23、TGF-β1水平，下调RORγt mRNA表达量。结论：人参皂苷Rg1呈剂量依赖性改善PM2.5致哮喘大鼠肺气道炎症反应、改善肺损伤。

English Summary:

Based on the airway IL-17/IL-23 inflammatory factor axis，to explore the protective mechanism of Ginsenoside Rg1 on lung injury in PM2.5 asthmatic rats.Methods:PM2.5 was isolated and sensitized by ovalbumin，and then PM2.5 was intratracheal instilled to establish PM2.5 asthmatic lung injury model.The levels of IL-17a，IL-6，IL-23 and TGF-β1 in serum and BALF were measured by ELISA.The expression of RORγt mRNA was detected by RT-PCR，and the lung tissue pathology and electron microscopy were analyzed.Results:The asthmatic rat model of PM2.5 was established successfully; The expression of IL-17a，IL-6，IL-23 and TGF-β1 in serum and BALF of asthmatic rats increased(P<0.01)，and the expression of RORγt mRNA in lung tissue increased(P<0.01); After PM2.5 intratracheal instillation，the contents of IL-17a，IL-6，IL-23 and TGF-β1 in serum and BALF of model group were further increased，and the expression of RORγt mRNA was further increased(P<0.01);After intervention with saponin Rg1，IL-17a，IL-6，IL-23，TGF-β1 in serum and BALF were decreased in a dose-dependent manner，and RORγt mRNA expression was down regulated.Conclusion:Ginsenoside Rg1 can improve airway inflammation and lung injury induced by PM2.5 in a concentration dependent manner.

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