| 引用本文:薛刚1，刘杰1，许利军1，石建1，凌博凡2.扶正消积胶囊对胃癌细胞BGC-823的增殖抑制及凋亡的影响[J].世界中医药,2021,(10):. |
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| 扶正消积胶囊对胃癌细胞BGC-823的增殖抑制及凋亡的影响 |
| Effect of Fuzheng Xiaoji Capsule on Proliferation Inhibition and Apoptosis of Gastric Cancer Cell BGC-823 |
| 投稿时间：2020-03-15 |
| DOI：10.3969/j.issn.1673-7202.2021.10.005 |
| 中文关键词: 扶正消积胶囊 BGC-823胃癌细胞 肿瘤 增殖 凋亡 |
| English Keywords:Fuzheng Xiaoji Capsule BGC-823 human gastric cancer cell Cancer Proliferation Apoptosis |
| 基金项目:国家中医药管理局基地开放课题（JD2019SZ05）；南通市青年医学人才项目（青年078） |
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| 中文摘要: |
| 目的：研究扶正消积胶囊在体外对人胃癌细胞BGC-823增殖抑制及凋亡的影响。方法：MTT实验检测扶正消积胶囊对BGC-823细胞增殖的影响；流式细胞仪检测其对BGC-823细胞凋亡的影响；Real Time-PCR、Western blotting实验检测其凋亡相关基因表达。结果：与空白组比较，BGC-823细胞的增殖能被扶正消积胶囊显著抑制（给药24 h），此抑制率随给药剂量的增加而上升，在剂量为5 mg/ mL时，其抑制率达86.71%，且这种抑制呈剂量依赖关系。Annexin V/PI双染法联合流式细胞仪结果显示扶正消积胶囊能诱导BGC-823细胞凋亡，BGC-823细胞早期凋亡率随给药剂量的增加，在给药剂量分别为2.5 mg/mL、5 mg/mL时，BGC-823细胞早期凋亡率分别达到15.76%、38.25%；Western blotting、Real Time-PCR结果表明，Procaspase-3、9蛋白酶表达随剂量的增加而明显减小，Bcl-2、Bcl-xl、Survivin 3种基因表达随给药剂量的增加而下降，Bax基因表达则相反。结论：扶正消积胶囊能通过激活caspase级联反应抑制胃癌细胞增殖并诱导其凋亡。 |
| English Summary: |
| To investigate the influence of Fuzheng Xiaoji Capsule on the proliferation inhibition of human gastric cancer cell BGC-823 and its apoptosis.Methods:Effects of Fuzheng Xiaoji Capsule on proliferation of BGC-823 cells were investigated with MTT assay.Flow cytometry was used to detect the cell apoptosis while the effect of apoptosis related gene of Fuzheng Xiaoji capsule on BGC-823 cell was measured by real time-PCR and Western blotting.Results:MTT assay showed that the BGC-823 cells were inhibited by Fuzheng Xiaoji Capsule compared with control group，after treated on 24 h.The inhibition rate increases with the increase of dose，which is dose dependent.At the dose of 5 mg/mL，the inhibition rate reached 86.71%(P<0.05).Flow cytometry demonstrated that Fuzheng Xiaoji Capsule could induce the apoptosis of BGC-823 cells.The early apoptosis rate of BGC-823 cells increased with the dose of the drug.At the dose of 2.5 mg/mL and 5 mg/mL，respectively，the early apoptosis of BGC-823 cells reached 15.76% and 38.25% respectively.By the results of Western blotting and real time-PCR，the expression of caspase-3、caspase-9 was significantly lower as the dose increased.Also，it could reduce the activity of Bcl-2，Bcl-xl，Survivin and increase the activity of Bax.Conclusion:Fuzheng Xiaoji Capsule could inhibit the proliferation of BGC-823 cells and induce their apoptosis.The possible mechanism was through activation of caspase cascade reaction. |
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