To explore the clinical application of acupuncture in the prevention and treatment of cancer-related fatigue，and to provide the basis for the development of clinical practice guidelines.Methods:We searched China national knowledge infrastructure(CNKI)，China Science Periodical Database(CSPD)，Chinese Citation Database(CCD)，Chinese Biological Literature，PubMed，EMBASE and Cochrane Library and other databases to comprehensively collect the modern literature evidence of acupuncture treatment of cancer-related fatigue; collected the ancient literature evidence of acupuncture treatment of cancer-related fatigue by manually searching 49 ancient medical books; collected the modern medical experts' experience of acupuncture intervention of cancer-related fatigue by formulating and distributing the Questionnaire of Acupuncture Intervention On Cancer-Related Fatigue.The 3 aspects of evidence were integrated to form the clinical recommended scheme of acupuncture in the prevention and treatment of cancer-related fatigue.Results:A total of 225 modern literature were retrieved，including 141 Chinese literature and 84 English literature.After sorting out the literature，14 modern literature were finally included; there were 10 items related to ancient medical literature; 52 clinical experts questionnaires were collected.After comprehensive 3 aspects of research results，it was found that acupuncture treatment of cancer-related fatigue commonly used acupuncture，moxibustion，acupoint pressing，electro acupuncture，transcutaneous acupoint electrical stimulation and other therapies; the commonly used acupoints were Zusanli(S36)，Sanyinjiao(SP6)，Guanyuan(CV4)，Qihai(CV6)，Taixi(K3)，Hegu(L14)，Zhongwan(CV12)，Baihui(GV20)，Taichong(LR3)，Yintang(EX-HN3)，Tianshu(S25).Conclusion:Based on the evidence of the 3 aspects，the recommended clinical application scheme of acupuncture for cancer-related fatigue prevention and treatment is formed.Acupuncture，moxibustion and acupoint pressing are strongly recommended; transcutaneous acupoint electrical stimulation and electroacupuncture are weakly recommended; Zusanli(S36)，Sanyinjiao(SP6)，Guanyuan(CV4)，Qihai(CV6)，Taixi(K3)，Hegu(L14)，Zhongwan(CV12)，Neiguan(P6)，Baihui(GV20)，Taichong(LR3)，Yintang(EX-HN3)，Tianshu(S25)，and Shenmen(TF4)etc.are recommended.