| 引用本文:陈逴凡1，李红毅2，杨贤平1，李玉清1.基于网络药理学的逍遥散治疗痤疮的作用机制[J].世界中医药,2021,(12):. |
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| 基于网络药理学的逍遥散治疗痤疮的作用机制 |
| Study on Mechanism of Xiaoyao Powder in Treatment of Acne Based on Network Pharmacology |
| 投稿时间：2020-06-18 |
| DOI：10.3969/j.issn.1673-7202.2021.12.001 |
| 中文关键词: 逍遥散 痤疮 中医药 网络药理 蛋白质 靶点 通路 作用机制 |
| English Keywords:Xiaoyao Powder Acne Traditional Chinese medicine Network pharmacology Protein Target Pathway Mechanism of action |
| 基金项目:国家中医药管理局中医学术流派传承工作室建设项目（LP0119045）——岭南皮肤病流派传承工作室；国家中医药管理局禤国维国医大师传承工作室 |
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| 中文摘要: |
| 目的：通过网络药理学方法探究逍遥散治疗痤疮的主要活性成分及其作用机制。方法：应用中药系统药理数据库与分析平台（TCMSP）筛选逍遥散中含有的有效成分及其作用靶标，并从基因表达综合数据库（GEO）中提取痤疮相关靶标。使用Cytoscape软件及其bisogenet插件构建逍遥散治疗痤疮的“化合物-靶点”网络，并绘制药物活性成分-靶蛋白-疾病蛋白质-蛋白质相互作用（PPI）网络，再分次进行拓扑分析，得出核心网络。采用R语言软件中ClusterProfiler数据包对逍遥散的潜在作用基因靶点进行基因本体（Gene Ontology,GO）富集分析和京都基因和基因组百科全书（Kyoto Encyclopedia of Genes and Genomes，KEGG）富集分析，并进行可视化作图。结果：通过TCMSP，获得了逍遥散有效活性成分161个，潜在作用靶点2 619个，与146个痤疮相关靶点的共同靶点为37个，包括PTGS2、CCNA2、CHEK1、NR3C2等，潜在活性成分为木犀草素、槲皮素、异鼠李素等110个。GO和KEGG富集分析结果显示，逍遥散治疗痤疮主要通过脂多糖应答、细菌源性分子应答等作用机制，与肿瘤坏死因子信号通路、IL-17信号通路、P53信号通路、核因子κB信号通路等相关。结论：本研究通过网络药理学的方法验证了逍遥散治疗痤疮的多成分、多靶点、多通路的作用机制特点，简单阐述了逍遥散治疗痤疮潜在有效成分及作用通路，为后续的相关研究提供理论支持。 |
| English Summary: |
| To investigate the main active components and mechanism of Xiaoyao Powder in the treatment of acne based on network pharmacology.Methods:The effective components and their targets in Xiaoyao Powder were screened by Pharmacological Database and Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP）,and acne related targets were extracted from gene expression database(GEO).The “compound target” network of Xiaoyao Pills was constructed by using the software of Cytoscape and bisogenet plugin.The PPI network of active ingredients,target proteins and diseases was drawn by using the software of Cytoscape and its bisogenet plug-in.The network topology analysis was carried out again,and the core network was obtained.Using ClusterProfiler data package in R language software,GO and KEGG analysis of potential gene targets of Xiaoyao Powder were performed and visualized mapping was performed.Results:A total of 161 active components and 2619 potential targets of Xiaoyao Powder were obtained from TCMSP.There were 37 common targets with 146 acne related targets,including PTGS2 CCNA2 CHEK1 NR3C2,etc.The 110 potential active ingredients were luteolin,quercetin,isorhamnetin,etc.The results of enrichment of GO and KEGG showed that the mechanism of Xiaoyao Powder in the treatment of acne was mainly concentrated in response to lipopolysaccharide and response to molecule of bacterial origin,mainly related to tumor necrosis factor signaling pathway,IL-17 signaling pathway,P53 signaling pathway,NF kappa B signaling pathway,etc.Conclusion:This study revealed the mechanism of Xiaoyao Powder in the treatment of acne through multi-component,multi-target and multi-channel,and makes a brief description of the potential effective ingredients and action pathways of Xiaoyao Powder in the treatment of acne,providing theoretical support for follow-up related research. |
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