| To explore the possible mechanism of action of Taohong Siwu Decoction in the treatment of rheumatoid arthritis by using network pharmacology and molecular docking technology, so as to provide theoretical basis for clinical application.Methods:TCMSP was used to retrieve the effective components and targets of Taohong Siwu Decoction, and disease-related targets were collected from GeneCards, OMIM, TTD, PharmGkb and DrugBank databases.Cytoscape 3.6.1 track software was used to build “compound-targets” network.With the help of the STRING intersection target protein interaction network, and Cytoscape was used for network topology analysis, and the core genes was obtained.By R language, GO enrichment and KEGG pathway enrichment analysis were conducted.Finally, molecular docking verification of core genes and main active ingredients performed molecular docking verification.Results:It was predicted that there were 41 active components and 154 corresponding targets in the treatment of RA by Taohong Siwu Decoction.Topological analysis revealed AKT1, JUN, TP53, MMP9, MAPK14 and other 10 core genes.GO enrichment analysis involved biological process(BP) 2 316, 75 cell components(CC), molecular function(MF) 205, KEGG pathway enrichment analysis filter to get 158 RA related pathways, mainly involving P13K-AKT, interleukin 17, tumor necrosis factor signaling pathways and other pathway, such as centralized control inflammation, pannus formation, cell proliferation and apoptosis, etc.The results of molecular docking showed that the main active compounds can bind to the core targets respectively and exhibit good affinity.Conclusion:In this study, the effect and mechanism of Taohong Siwu Decoction in the treatment of RA with multiple components, multiple targets and multiple pathways were preliminarily discussed, so as to provide reference for subsequent basic research and theoretical basis for clinical application. |
