| 引用本文:范婷婷1，万彩云1，卢青青1，荆晓朔1，张伟2，范瑜洁3，聂颖4，李亮1.补阳还五汤改善脊髓损伤的抗氧化功能及作用机制[J].世界中医药,2021,(12):. |
|
| 补阳还五汤改善脊髓损伤的抗氧化功能及作用机制 |
| Improve the Antioxidant Function and Action Mechanism of Spinal Cord Injury by Buyang Huanwu Decoction |
| 投稿时间：2020-11-25 |
| DOI：10.3969/j.issn.1673-7202.2021.12.001 |
| 中文关键词: 补阳还五汤 脊髓损伤 气虚血瘀证 氧化应激 神经营养因子 红核脊髓束 超氧化物歧化酶 丙二醛 |
| English Keywords:Buyang Huanwu Decoction Spinal cord injury Qi-deficiency and blood-stasis syndrome Antioxidation Brain derived neurotrophic factor Rubrospinal tract Superoxide dismutase Malondialdehyde |
| 基金项目:国家自然科学基金项目（81302899）——基于cAMP/PKA/RhoA信号通路探讨益气活血法促进BMSCs移植修复脊髓损伤的机制；湖南省自然科学基金项目（2018JJ2289）——补阳还五汤介导钙超载修复脊髓损伤的机制研究；湖南省自然科学基金项目（2019JJ80071）——基于红核蛋白质组学探讨益气活血法修复脊髓损伤的分子机制；湖南省自然科学基金项目（2019JJ50310）——基于mTOR通路探讨益气活血法对脊髓损伤大鼠红核自噬的调节机制；湖南中医药大学中医学一流学科开放基金项目（2018ZYX01）——基于线粒体能量代谢异常探讨益气活血法挽救脊髓损伤凋亡细胞的机制 |
|
| 摘要点击次数: 711 |
| 全文下载次数: 0 |
| 中文摘要: |
| 目的：探讨补阳还五汤（BYHWD）对脊髓损伤（SCI）大鼠的抗氧化作用及其机制。方法：将24只雄性SD大鼠随机分为对照组、SCI组、BYHWD低剂量（BL）组、BYHWD高剂量（BH）组，每组6只。采用游泳力竭法联合红核脊髓束（RST）横断手术制备SCI气虚血瘀证大鼠模型，对照组和SCI组予以生理盐水灌胃，其他组予以BYHWD灌胃，1次/d。通过自发性直立探究行为（前肢使用率）实验测试运动功能恢复情况，酶联免疫吸附试验（ELISA）法检测血清中丙二醛（MDA）和超氧化物歧化酶（SOD）水平，激光共聚焦观察环磷酸腺苷反应元件结合蛋白/脑源性神经营养因子(CREB/BDNF)的表达。结果：干预后，BH组前肢和右前肢的利用率高于SCI组和BL组（P<0.05或P<0.01）；与SCI组比较，观察组的MDA水平均下降，SOD水平均上升，以BH组水平变化最为明显，差异有统计学意义（P<0.05或P<0.01）；激光共聚焦法显示，与SCI组比较，观察组CREB、BDNF的表达均上升，以BH组表达变化最为明显，差异有统计学意义（P<0.05或P<0.01）。结论：补阳还五汤可改善SCI大鼠的抗氧化功能，其机制涉及CREB/BDNF信号通路的调节。 |
| English Summary: |
| To explore the effects and the underlying mechanism of Buyang Huanwu Decoction(BYHWD) antioxidant function in spinal cord injury rats.Methods:A total of 24 male rats were randomly divided into the following 4 groups:a control group,a spinal cord injury model group(SCI group),a low-dose of BYHWD group(BL group),a high-dose of BYHWD group(BH group),with 6 rats in each group.The rat model was established by swimming exhaustion combined with the transection of rubrospinal tract(RST).The rats in control group and SCI group were given normal saline by gavage once,and the other groups were given BYHWD by gavage once.The recovery of motor function was observed by spontaneous vertical exploration test.MDA and SOD in serum was detected by ELISA.laser scanning confocal microscopy was adapted to detect the expression of CREB and BDNF.Results:Compared with the SCI group,the utilization rates of both forelimbs and the right forelimb of the BH group were higher than those of the SCI group and the BL group(P<0.05 or P<0.01).Compared with the SCI group,ELISA showed that the expression of MDA were decreased in the treatment group,while the expression of SOD was increased,in which the BH group were most significant(P<0.05 or P<0.01)；Compared with the SCI group,Laser scanning confocal microscopy showed that the expression of CREB and BDNF were increased,in which the BH group were most significant(P<0.05 or P<0.01).Conclusion:BYHWD could improve the antioxidant function through the CREB/BDNF pathway in spinal cord injury rats. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
