To explore the protective effect of Huqian Pill on bone tissue of ovariectomized osteoporosis rats and the mechanism of prevention and treatment of osteoporosis by regulating the expression of ALP，IL-6 and OPG.Methods:The model of postmenopausal osteoporosis rats was made by ovariectomy.The rats were randomly divided into a model group，a Huqian Pills group and an estradiol group.At the same time，a sham operation group was set up as a control，and each group was given corresponding drugs by gavage.After 12 weeks of gavage，blood was taken and executed，the body mass and uterus mass of each group were weighed; the bone density of left femur and tibia of each group were measured; the changes of blood glucose，blood lipid and calcium phosphorus in serum of each group were detected by automatic biochemical analyzer; the contents of IL-6，ALP and OPG in rat serum of each group were detected by ELISA.Results:Compared with sham group，model group BMD decreased significantly(P<0.05); there was significant destruction of bone microarchitecture; compared with model group，Huqian Pill increased bone density of rats，increases the integrity of trabecular bone，bone microarchitecture was improved，the amount of weight gain decreased，blood lipid and glucose levels decreased，IL-6，and ALP content were decreased，levels of OPG elevated，calcium content significantly increased.The differences were statistically significant(P<0.05).Conclusion:Haqian Pills can improve bone mass in ovariectomized rats，prevent postmenopausal osteoporosis，and its mechanism may reduce ALP，IL-6，and improve OPG related content.