| 引用本文:王进1，张倩1,2，唐好知1，杨旭1，王花欣1,2.虎潜丸对去卵巢骨质疏松症大鼠骨组织的保护作用及其机制研究[J].世界中医药,2021,(12):. |
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| 虎潜丸对去卵巢骨质疏松症大鼠骨组织的保护作用及其机制研究 |
| Study on the Protective Effect and Mechanism of Huqian Pill on Bone Tissue of Ovariectomized Osteoporosis Rats |
| 投稿时间：2020-07-08 |
| DOI：10.3969/j.issn.1673-7202.2021.12.001 |
| 中文关键词: 虎潜丸 绝经后骨质疏松症 骨密度 去卵巢大鼠 雌激素 白细胞介素-6 中医药治疗 |
| English Keywords:Huqian Pills Postmenopausal osteoporosis Bone density Ovariectomized rats Estrogen IL-6 Traditional Chinese medicine treatment |
| 基金项目:国家自然科学基金委员会青年科学基金项目（82004087）；山东省中医药科技发展计划项目（2017-004）；山东省高等学校科技计划项目（J18KA271）；山东省自然科学基金重大基础研究项目（ZR2019ZD23）；国家级大学生创新训练计划项目（201910441010）；山东中医药大学青年创新团队项目 |
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| 中文摘要: |
| 目的:探讨虎潜丸对去卵巢骨质疏松症大鼠骨组织的保护作用，以及通过调控碱性磷酸酶(ALP)、白细胞介素-6（IL-6）和血清骨保护素(OPG)的表达而防治骨质疏松症的机制研究。方法:采用卵巢切除法复制绝经后骨质疏松症大鼠模型，随机分为模型组、虎潜丸组、雌二醇组，同时设置假手术组作为对照，各组分别给予相应的药物灌胃。灌胃12周后取血处死，称量各组大鼠的体质量、子宫重量；取大鼠左侧股骨、胫骨进行骨密度检测；全自动生化分析仪检测各组大鼠血清中血糖、血脂、钙磷的变化；ELISA检测各组大鼠血清中IL-6、ALP、OPG的水平。结果:与假手术组比较，模型组大鼠骨密度显著下降(P<0.05)，骨微结构破坏明显；与模型组比较，虎潜丸组大鼠骨密度增加，骨小梁的完整性升高，骨微结构得到改善，体质量增长明显下降，血脂血糖水平降低，IL-6、ALP含量均有所降低，OPG含量升高，血钙含量有明显上升，差异有统计学意义(P<0.05)。结论:虎潜丸能够提高去卵巢大鼠的骨量，防治绝经后骨质疏松症，其作用机制可能与降低ALP、IL-6，提高OPG含量有关。 |
| English Summary: |
| To explore the protective effect of Huqian Pill on bone tissue of ovariectomized osteoporosis rats and the mechanism of prevention and treatment of osteoporosis by regulating the expression of ALP，IL-6 and OPG.Methods:The model of postmenopausal osteoporosis rats was made by ovariectomy.The rats were randomly divided into a model group，a Huqian Pills group and an estradiol group.At the same time，a sham operation group was set up as a control，and each group was given corresponding drugs by gavage.After 12 weeks of gavage，blood was taken and executed，the body mass and uterus mass of each group were weighed; the bone density of left femur and tibia of each group were measured; the changes of blood glucose，blood lipid and calcium phosphorus in serum of each group were detected by automatic biochemical analyzer; the contents of IL-6，ALP and OPG in rat serum of each group were detected by ELISA.Results:Compared with sham group，model group BMD decreased significantly(P<0.05); there was significant destruction of bone microarchitecture; compared with model group，Huqian Pill increased bone density of rats，increases the integrity of trabecular bone，bone microarchitecture was improved，the amount of weight gain decreased，blood lipid and glucose levels decreased，IL-6，and ALP content were decreased，levels of OPG elevated，calcium content significantly increased.The differences were statistically significant(P<0.05).Conclusion:Haqian Pills can improve bone mass in ovariectomized rats，prevent postmenopausal osteoporosis，and its mechanism may reduce ALP，IL-6，and improve OPG related content. |
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