| 引用本文:谭晖1，王吉昌2，董丹凤3，李恩孝3，李毅3.五味子酯甲通过抑制CCAT1和PI3K-AKT信号通路抑制肺癌细胞的迁移和侵袭[J].世界中医药,2021,(13):. |
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| 五味子酯甲通过抑制CCAT1和PI3K-AKT信号通路抑制肺癌细胞的迁移和侵袭 |
| Schisantherin A Inhibits the Migration and Invasion of Lung Cancer Cells by Inhibiting the CCAT1 and PI3K-AKT Signaling Pathway |
| 投稿时间：2020-11-19 |
| DOI：10.3969/j.issn.1673-7202.2021.13.009 |
| 中文关键词: 五味子酯甲 肺癌 迁移 侵袭 增殖 凋亡 结肠癌相关转录本-1 PI3K/AKT信号通路 |
| English Keywords:Schisantherin A Lung cancer Migration Invasion Proliferation Apoptosis CCAT1 PI3K/AKT signaling pathway |
| 基金项目:国家自然科学基金项目（81972811）；陕西省重点研发计划项目（2020SF-029） |
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| 中文摘要: |
| 目的：探究五味子酯甲（SA）对肺癌细胞生长和转移的潜在作用机制。方法：本研究以非小细胞肺癌细胞系A549和H1299作为实验对象，将SA溶解在二甲基亚砜中，用不同剂量的SA（0 μmol/L、5 μmol/L、10 μmol/L、20 μmol/L、50 μmol/L）孵育A549和H1299细胞24 h、48 h和72 h。采用细胞计数盒-8检测细胞增殖，采用FC500流式细胞仪分析细胞凋亡，采用伤口愈合实验评价细胞迁移，采用Transwell检测细胞侵袭。通过实时定量逆转录聚合酶链反应（qRT-PCR）检测细胞中CCAT1 mRNA水平；通过Western Blotting检测细胞中p-PI3K、PI3K、p-AKT、AKT、PTEN、E-cadherin、MMP2和MMP9的蛋白表达水平。结果：与未处理的细胞比较，SA以剂量和时间依赖性方式降低了A549和H1299的细胞活力（P<0.05）。与DMSO组比较，SA 10 μmol/L组的A549和H1299细胞凋亡率显著升高了5.71倍和3.48倍（P<0.001），伤口愈合面积显著降低了63.42%和59.15%（P<0.001），侵袭细胞数显著降低了53.98%和53.36%（P<0.001）。与DMSO组比较，SA 10 μmol/L组A549和H1299细胞中E-cadherin的蛋白表达水平升高，而MMP-2和MMP-9的蛋白表达水平降低（P<0.01），CCAT1的mRNA表达水平均降低（P<0.01），p-PI3K和p-AKT的蛋白表达水平显著降低（P<0.01），PTEN的蛋白表达水平显著升高（P<0.01），而PI3K和AKT蛋白表达水平无明显变化（P>0.05）。结论：SA通过抑制CCAT1和PI3K/AKT信号通路抑制肺癌细胞的生长和转移。 |
| English Summary: |
| To explore the potential effect and mechanism of Schisantherin A(SA) on the growth and metastasis of lung cancer cells.Methods:In this study，the non-small cell lung cancer cell lines A549 and H1299 were used as experimental objects.SA was dissolved in dimethyl sulfoxide(DMSO)，and A549 and H1299 was incubated with different concentrations of SA(0 μM，5 μM，10 μM，20 μM，50 μM) for 24 h，48 h and 72 h.Then，Cell Counting Kit-8(CCK-8) was used to detect cell proliferation，FC500 flow cytometer was used to analyze cell apoptosis，wound healing experiment was used to evaluate cell migration，and Transwell was used to detect cell invasion.Real-time quantitative reverse transcription polymerase chain reaction(qRT-PCR) was used to detect CCAT1mRNA levels in cells.Western blot was used to detect the protein expression levels of p-PI3K，PI3K，p-AKT，AKT，PTEN，E-cadherin，MMP2 and MMP9 in cells.Results:Compared with untreated cells，SA reduced the cell viability of A549 and H1299 in a concentration and time-dependent manner(P<0.05).Compared with the DMSO group，the apoptosis rate of A549 and H1299 cells in the SA 10 μM group was significantly increased by 5.71 times and 3.48 times(P<0.001)，the wound healing area of A549 and H1299 cells in the SA 10 μM group was significantly reduced by 63.42% and 59.15%(P<0.001)，and the number of invasion cells of A549 and H1299 cells in the SA 10 μM group was significantly reduced by 53.98% and 53.36%(P<0.001).Compared with the DMSO group，the protein expression levels of E-cadherin in A549 and H1299 cells in the SA 10 μM group increased，while the protein expression levels of MMP-2 and MMP-9 decreased(P<0.01); the mRNA and protein expression levels of CCAT1 in A549 and H1299 cells in the SA 10 μM group were lower(P<0.01); the protein expression of p-PI3K and p-AKT in A549 and H1299 cells in SA 10 μM group decreased significantly(P<0.01)，PTEN protein expression level increased significantly(P<0.01)，while PI3K and AKT protein expression levels did not change significantly(P>0.05).Conclusion:SA inhibits the growth and metastasis of lung cancer cells by inhibiting the CCAT1 and PI3K/AKT signaling pathways. |
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