| 引用本文:申萌萌1，刘雁峰2，周雨玫2，史亚婷2，朱庆文3.二补助育汤对着床障碍小鼠子宫环氧化酶-2、过氧化物酶体增殖物激活受体δ的影响[J].世界中医药,2021,(14):. |
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| 二补助育汤对着床障碍小鼠子宫环氧化酶-2、过氧化物酶体增殖物激活受体δ的影响 |
| Effects of Erbu Zhuyu Decoction on Uterine Cyclooxygenase-2 and Peroxisome Proliferator Activated Receptor δ for Mice with Embryo Implantation Dysfunction |
| 投稿时间：2020-06-19 |
| DOI：10.3969/j.issn.1673-7202.2021.14.009 |
| 中文关键词: 二补助育汤 着床障碍 吲哚美辛 小鼠 子宫形态 环氧化酶-2 过氧化物酶体增殖物激活受体δ 子宫内膜容受性 |
| English Keywords:Erbu Zhuyu Decoction Model of embryo implantation dysfunction Indomethacin Mouse Uterus histomorphology COX-2 PPARδ Endometrial receptivity |
| 基金项目:国家自然科学基金项目(81904242)；北京中医药大学校级课题(2019-JYB-XJSJJ041) |
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| 中文摘要: |
| 目的:探讨二补助育汤对着床障碍小鼠内膜中环氧化酶-2(COX-2)、过氧化物酶体增殖物激活受体δ(PPARδ)表达的影响。方法:将C57妊娠小鼠随机分为空白、模型、阿司匹林、戊酸雌二醇片、二补助育汤高、中、低剂量组,每组8只,吲哚美辛油制备胚胎着床障碍小鼠模型,各组给予相应药物灌胃。妊娠第5天取小鼠子宫,观察外观形态、COX-2、PPARδ基因和蛋白表达。结果:模型组小鼠子宫苍白细长,串珠样改变少,且分布稀疏不均。各观察组小鼠子宫较模型组红润,有串珠状改变,串珠分布增多且不均。模型组COX-2和PPARδ蛋白表达低于空白组(P<0.05);各观察组COX-2蛋白表达均高于模型组(均P<0.05)。二补助育汤低、高剂量组PPARδ mRNA表达较模型组、戊酸雌二醇片组明显增加(P<0.05),阿司匹林组PPARδ mRNA表达高于二补助育汤中剂量组(P<0.05);二补助育汤各剂量组PPARδ蛋白表达高于模型组(P<0.01),其中中剂量组高于戊酸雌二醇片组和阿司匹林组(P<0.05)。结论:二补助育汤能够调节子宫内膜COX-2和PPARδ表达,提高子宫内膜容受性,有利于胚胎着床。 |
| English Summary: |
| To explore the effects of Erbu Zhuyu Decoction on cyclooxygenase-2 (COX-2) and peroxisome proliferators-activated receptors δ (PPARδ) expression in mouse model of embryo implantation dysfunction.Methods:A C57 pregnant mice were randomly divided into a blank group,a model group,an aspirin group,a progynova group,a high-dose,a middle-dose and a low-dose groups,with 8 cases in each group.Embryo implantation dysfunction model was built with indomethacin.Mice in each group were administrated with corresponding medicine.The uteri were excised on 5th day of pregnancy to determine the expression of COX-2 and PPARδ.Results:The uteri in model group showed pale and slender.Beads changed little,and the distribution was sparse and uneven.Compared with the model group,the uterus of mice in each treatment group was rosier,with bead-like changes,and the distribution of beads was increased and uneven.The expression of COX-2 and PPARδ protein in the model group was lower than that in the blank group (P<0.05); the expression of COX-2 protein in each treatment group was higher than that in the model group (all P<0.05).The expression of PPARδ mRNA in the low-dose and high-dose groups of Erbu Zhuyu Decoction was significantly higher than that of the model group and estradiol valerate tablets group (P<0.05),and the expression of PPARδ mRNA in the aspirin group was higher than that of the middle-dose Erbu Zhuyu group (P<0.05).The expression of PPARδ protein in each dose group of Erbu Zhuyu was higher than that of the model group (P<0.01),and the middle dose group was higher than that of estradiol valerate tablets group and aspirin group (P<0.05).Conclusion:Erbu Zhuyu Decoction could improve endometrial receptivity by regulating COX-2 and PPARδ expression,improve the receptivity of the endometrium and be conducive to embryo implantation. |
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