| 引用本文:周英奕1，周梦玲2，田金洲1，魏明清1，倪敬年1，时晶1.APP/PS1小鼠肠道菌群特点及补肾法干预对其的影响[J].世界中医药,2021,(14):. |
|
| APP/PS1小鼠肠道菌群特点及补肾法干预对其的影响 |
| Study on the Characteristics of APP/PS1 Mice Intestinal Flora and the Effects of the Intervention of the Kidney-tonifying Method on It |
| 投稿时间：2020-09-01 |
| DOI：10.3969/j.issn.1673-7202.2021.14.011 |
| 中文关键词: 阿尔茨海默病 APP/PS1转基因小鼠 细菌 肠道菌群 菌群失调 16SrDNA技术 补肾法 中药 |
| English Keywords:Alzheimer's disease APP/PS1 transgenic mice Bacteria Gut microbiota Dysbacteriosis 16srDNA detection Kidney-tonifying method Chinese medicinal |
| 基金项目:教育部长江学者和创新团队发展计划项目(IRT0810);中央高校基本科研业务费专项(2019-JYB-TD-007;2019-JYB-JS-066);中医药传承与创新“百千万”人才工程(岐黄工程)岐黄学者 |
|
| 摘要点击次数: 608 |
| 全文下载次数: 0 |
| 中文摘要: |
| 目的:探究不同月龄APP/PS1双转基因模型小鼠的肠道菌群特点及补肾法对其肠道菌群失衡的影响。方法:将8只6个月龄雄性APP/PS1小鼠随机分为模型组和补肾组;4只6个月龄雄性C57BL/6小鼠作为正常对照组。补肾组小鼠给予补肾法中药灌胃3个月,模型组和正常对照组小鼠均予以等体积羧甲基纤维素(CMC)溶液灌胃。留取小鼠灌胃干预前后的粪便,运用16SrDNA技术检测各组肠道菌群的组成结构。从肠道微生态方面,探讨补肾中药对APP/PS1双转基因小鼠肠道菌群构成的影响。结果:6个月龄时模型组和正常对照组小鼠的肠道菌群差异无统计学意义;与9个月龄正常组比较,模型组小鼠肠道内芽孢杆菌纲丰度明显升高。与模型组比较,补肾组肠道内芽孢杆菌纲丰度明显下降,疣微菌门丰度呈上升趋势。结论:APP/PS1小鼠体内均存在肠道菌群失衡的情况,主要与芽孢菌纲、疣微菌门的丰度水平的变化相关。补肾法可以通过降低芽孢菌纲、升高疣微菌门的丰度来进一步改善AD小鼠肠道菌群失衡状态。 |
| English Summary: |
| To explore the characteristics of gut microbiota in APP/PS1 transgenic mice at different ages and the effect of tonifying spleen method on its intestinal flora imbalance.Methods:Eight 6-month-old male APP/PS1 mice were randomly divided into a model group and a kidney-tonifying group，and four 6-month-old male C57BL/6 mice were served as normal control group.The mice in the kidney-tonifying group were treated with Chinese medicinal for kidney-tonifying spleen for 3 months，and the mice in the model group and normal control group were given CMC solution.The feces of mice before and after gavage intervention were collected，and the composition and structure of gut microbiota in each group were detected by 16S rDNA technology.From the aspect of intestinal microecology，the effects of kidney-tonifying Chinese medicinal on gut microbiota of APP/PS1 transgenic mice were investigated.Results:At the age of 6 months，there was no significant difference in gut microbiota between the model group and the normal control group.Compared with the normal group at the age of 9 months，the abundance of Bacilli in the intestinal tract of the model group was significantly higher than normal group.Compared with the model group，the abundance of Bacilli was significantly decreased，and the abundance of Verrucomicrobia in the kidney-tonifying group was increased.Conclusion:The imbalance of gut microbiota was found in APP/PS1 mice，which was mainly related to the changes of the abundance of Bacilli and Verrucomicrobia.Kidney-tonifying method can improve the imbalance of gut microbiota by reducing the abundance of Bacilli and increasing the abundance of Verrucomicrobia. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
