| 引用本文:吴思骐1，刘建博2.慢性阻塞性肺疾病差异基因与中药预测的生物信息学分析[J].世界中医药,2021,(14):. |
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| 慢性阻塞性肺疾病差异基因与中药预测的生物信息学分析 |
| Bioinformatics Analysis of Differential Genes in Chronic Obstructive Pulmonary Disease and Prediction of Chinese Medicinal |
| 投稿时间：2020-10-26 |
| DOI：10.3969/j.issn.1673-7202.2021.14.023 |
| 中文关键词: 差异表达基因 慢性阻塞性肺疾病 微小RNA 中药 香烟烟雾 |
| English Keywords:Differentially expressed gene Chronic obstructive pulmonary disease miRNA Chinese medicinal Cigarette smoke |
| 基金项目:刘小虹广东省名中医传承工作室建设项目(粤中医办函【2018】5号);广州市科技计划项目(201803010053) |
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| 中文摘要: |
| 目的:通过生物信息学技术分析比较慢性阻塞性肺疾病(COPD)患者与健康人的基因芯片数据,初步筛选COPD的差异表达基因,并借助差异表达基因预测治疗COPD的潜在中药药物。方法:首先通过R分析GSE27597和GSE106986基因数据,得到139个差异表达基因,对差异表达基因进行基因本体(GO)富集分析,并通过String数据库、Cytoscape软件及其插件分析得到14个核心基因,然后对核心基因进行Reactome、KEGG、PANTHER等通路注释,最后通过核心基因与医学本体信息检索平台(Coremine Medical)相互映射,筛选治疗COPD的中药。结果:通过MCODE筛选出14个核心基因包括MMP8、PTX3、DEFA4、CHRM2、FPR2、CX3CR1、FPR1、ORM1、APLNR、PPBP、CHIT1、AGTR2、ARG1,与中性粒细胞脱颗粒、先天免疫系统、趋化因子信号通路等密切相关,其中FPR1和CX3CR1通过影响中性粒细胞向肺部的迁移活动成为COPD病理变化的关键。治疗COPD的潜在中药药物包括黄连、黄芪、黄芩、半夏、丹参、厚朴、姜黄、郁金、瓜蒌等。结论:部分基因会促进香烟烟雾暴露诱发COPD的发生,通过miRNA进行调节可能是中药治疗COPD的潜在作用机制。 |
| English Summary: |
| By analyzing and comparing the gene chip data of chronic obstructive pulmonary disease (COPD) patients and healthy people through bioinformatics technology,the differentially expressed genes of COPD are initially screened,and the potential Chinese medicinal drugs for the treatment of COPD are predicted with the help of differentially expressed genes.Methods:The gene expression profiles of lung tissues of COPD cases and healthy cases were obtained from GSE27597 and GSE106986 of the Gene Expression Omnibus.A otal of 139 differentially expressed genes were obtained,and we performed gene ontology (GO) enrichment analysis of differentially expressed genes,and obtained 14 core genes through String database,Cytoscape software and its plug-in analysis,and then we performed Reactome,KEGG,PANTHER and other pathways on the core genes.Finally through the mutual mapping between core genes and the medical ontology information retrieval platform (Coremine Medical),we screened Chinese medicinal for the treatment of COPD.Results:A total of 14 core genes screened by MCODE including MMP8,PTX3,DEFA4,CHRM2,FPR2,CX3CR1,FPR1,ORM1,APLNR,PPBP,CHIT1,AGTR2,ARG1,and neutrophil degranulation,innate immune system,chemokines signal pathways were closely related,among which FPR1 and CX3CR1 have become the key to the pathological changes of COPD by affecting the migration of neutrophils to the lungs.Potential Chinese medicinals for the treatment of COPD include Rhizoma Coptidis,Radix Astragali seu Hedysari,Radix Scutellariae,Rhizoma Pinelliae,Radix Salviae Miltiorrhizae,Cortex Magnoliae Officinalis,Rhizoma Curcumae Longae,Radix Curcumae,Fructus Trichosanthis.Conclusion:Some genes can promote the occurrence of COPD induced by cigarette smoke exposure,and regulation by miRNA may be a potential mechanism of Chinese medicinal in the treatment of COPD. |
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