世界中医药

目的：研究酸橙枳实中黄酮类抗氧化药效组分。方法：利用统计学中偏最小二乘法回归方法，将酸橙枳实黄酮类组分HPLC共有峰峰面积和用清除DPPH自由基、清除ABTS自由基、总抗氧化能力测定（FRAP）3个抗氧化活性指标结果进行回归分析，得出参与抗氧化作用的药效组分及各组分与抗氧化活性的相关性。用十批药材各药效组分的峰面积平均值计算药效组分之间比例。结果：酸橙枳HPLC图谱中的11个黄酮类组分峰与其抗氧化活性相关。与清除DPPH自由基正相关的药效组分,相关性由大到小分别是:新橙皮苷＞X1＞野漆树苷＞柚皮苷＞X6＞X3，组分间比例为138:34:1:149:15:12；与清除ABTS自由基正相关的药效组分，相关性由大到小分别是:新橙皮苷＞野漆树苷＞X1，组分间比例为138:1:34；与总抗氧化能力正相关的药效组分，相关性由大到小分别是:新橙皮苷＞X1＞柚皮苷＞X3＞川陈皮素＞橙皮苷＞X6＞芸香柚皮苷＞X4＞野漆树苷＞X2，组分间比例为138:34:149:12:19:12:15:13:08:1:04。结论：酸橙枳实抗氧化药效效应是多个黄酮类组分协同作用的结果；其中新橙皮苷、野漆树苷和未知成分X1是酸橙枳实黄酮类抗氧化核心药效组分；新橙皮苷、野漆树苷和未知成分X1之间的比例为138:1:34。

English Summary:

To study antioxidant active components alignment of flavonoids from aurantii fructus immaturus. Methods:By using the partial least squares regression method in statistics, the HPLC shared peak area of the flavonoids of citrus aurantium and the 3 antioxidant activities of scavenging DPPH free radicals, scavenging ABTS free radicals, and total antioxidant capacity(FRAP) were determined. Regression analysis was performed on the index results, and the pharmacodynamic components involved in the antioxidant effect and the correlation between each component and the antioxidant activity were obtained. The ratio between the effective components of the 10 batches of medicinal materials was calculated by using the average peak area of each effective component of the medicinal materials.Results:A total of 11 active components of flavonoids were relevant with antioxidant activity. The positive correlation of the pharmacodynamic components with DPPH radical scavenging was as follows：new hemperidin>X1>naringin>X6>X3, and the ratio was 13.8:3.4:1:14.9:1.5:1.2. For the active components positively correlated with ABTS free radical scavenging, the correlation from large to small was as follows：neohemperidin>wild sumacin>X1, with the ratio of 13.8:3.4:1:14.9:1.5:1.2. The positive correlation between the pharmacoactive components and total antioxidant capacity was as follows：neohiresperidin>X1>naringin>anacardin>naringin>X6>X3, the ratio of the components was 13.8:3.4:1:14.9:1.5:1.2; the effective components that were positively related to the scavenging of ABTS free radicals, the correlations from large to small were：neohesperidin＞rhosperidin＞X1, the ratio of the components was 13.8:1:3.4; the effective components that were positively related to the total antioxidant capacity, the correlations from large to small were：neohesperidin>X1>naringin>X3>Chrysanthin>Hesperidin>X6>Rutinnaringin>X4>Rhosoprin>X2, the ratio of the components was 13.8:3.4:14.9:1.2:1.9:1.2:1.5:1.3:0.8:1:0.4. Conclusion:The antioxidant active of Aurantii Fructus Immaturus is the results of synergisticeffect from many kinds of Flavonoids. Among them, neohesperidin, anacardin and unknown component X1 are the core anti-oxidant components of Lime Citrus aurantium flavonoids; the ratio of neohesperidin, anacardin and unknown ingredient X1 is 13.8:1:3.4.

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