To study the target and signal pathway of Epimedium-Morinda in the treatment of erectile dysfunction，and to explore the potential mechanism of Epimedium-Morinda in the treatment of erectile dysfunctionMethods:The active components and targets of epimedium-Morinda were screened out from TCMSP and BATMAN-TCM database，the disease targets of erectile dysfunction were obtained by Genecards，OMIM and NCBI gene human database，and the common targets of drugs and diseases were screened by venntuThe PPI network was constructed by using String database，and the topological analysis was carried out by using Cystoscap 372The intersection network of traditional Chinese medicine，active ingredient，disease and target was constructedThe common target was analyzed by using R363 software for GO analysis and KEGG pathway enrichment analysis，and the potential signal pathway was screened and its mechanism was analyzedResults:1）Morinda has 20 chemical constituents and 43 targetsEpimedium has 23 chemical constituents and 202 targetsAfter merging and deleting，203 targets are obtainedEffective ingredients include quercetin，kaempferol，sitosterol，luteolin，etc2）1004 targets were selected for erectile dysfunction3）There were 75 intersection targets of Epimedium-Morinda and erectile dysfunction4）A total of 30 key targets such as IL6，VEGFA and CASP3 were selected through PPI network5）The biological process of GO analysis was mainly concentrated in the reaction to lipopolysaccharide，the reaction to bacterial source molecules，the vascular reaction in the circulatory system,etc; the cell components were mainly concentrated in the membrane raft，membrane micro area，membrane area，presynaptic membrane and posterior membrane，etc; the molecular function was mainly concentrated in the activity of adrenergic receptor，G protein coupled amine receptor，cytokine activity，nuclear receptor，transcription factor activity，etc6）KEGG analysis enriched 17 signaling pathways，including PI3K AKT signaling pathway，neuroactive ligand receptor interaction，calcium signaling pathway，NF kappa B signaling pathway，cytokine cytokine receptor interaction，HIF-1 signaling pathway，p53 signaling pathway，cGMP-PKG signaling pathwayConclusion:Epimedium-Morinda plays an important role in the treatment of erectile dysfunction by affecting PI3K AKT and other signaling pathways，and may be related to the mechanisms of reducing the activities of arginase，acetylcholinesterase and adenosine deaminase，increasing the level of NO，inhibiting the activity of PDE-5 and affecting the cell signaling.