| 引用本文:王欢1，丁海涛1，舒骏1，王瑞茵2，齐蕊涵2，史榕荇1，杨帆1，唐学章1.舒筋活络方对骨关节炎患者滑膜成纤维细胞增殖、迁移和侵袭的影响[J].世界中医药,2021,(15):. |
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| 舒筋活络方对骨关节炎患者滑膜成纤维细胞增殖、迁移和侵袭的影响 |
| Effects of Shujin Huoluo Formula on the Proliferation，Migration and Invasion of Fibroblast-like Synovicytes in Osteoarthritis |
| 投稿时间：2020-03-26 |
| DOI：10.3969/j.issn.1673-7202.2021.15.017 |
| 中文关键词: 舒筋活络方 中药复方制剂 增殖 迁移 侵袭 滑膜成纤维细胞 骨关节炎 滑膜 |
| English Keywords:Shujin Huoluo Formula Chinese medicine compound preparation Proliferation Migration Invasion Fibroblast-like synovicytes Osteoarthritis Synovial |
| 基金项目:国家自然科学基金青年项目（81804214）；北京市优秀人才培养资助项目（2018000052580G469） |
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| 中文摘要: |
| 目的：测定舒筋活络方（SHC）对骨关节炎（OA）患者滑膜成纤维细胞（FLS）增殖、迁移、侵袭的影响，明确其作用机制。方法：对胶原酶法分离培养的FLS进行形态学观察，通过MTT比色法、划痕实验、Transwell实验、ELISA法等技术测定SHC对FLS增殖、迁移、侵袭等的影响。结果：体外分离的FLS生长状态良好，具备成纤维细胞形态特性；所选药物浓度范围对FLS增殖影响较小，与空白组比较，较高浓度和低浓度的SHC对FLS增殖的影响差异均有统计学意义（P<005），FLS的增殖与SHC呈浓度依赖性；镜下观察到OA-FLS的迁移现象，其中，模型组48 h、72 h的迁移率与0 h时比较，差异有统计学意义（P<005），SHC组72 h的迁移率与0 h时比较差异有统计学意义（P<005），与模型组比较，48 h、72 h时，SHC组FLS迁移能力比较，差异有统计学意义（P<005）；SHC组FLS侵袭穿过小室膜的细胞个数较空白组明显减少（P<005）；SHC各浓度组均对MMP3的释放有不同程度的抑制作用（P<005）。结论：SHC调节FLS增殖、抑制FLS的迁移和侵袭，维护和修复OA患者关节内组织完整性，为OA的关节保护提供新的预防和治疗策略。 |
| English Summary: |
| To determine the effects of Shujin Huoluo Formula(SHC) on the proliferation，migration and invasion of fibroblast-like synovicytes(FLSs) in patients with osteoarthritis(OA)，and to further clarify the mechanism of its action in the treatment of OAMethods:the FLSs isolated and cultured by collagenase were observed morphologically，and the effects of SHC on FLSs proliferation，migration and invasion were determined by MTT colorimetry，scratch assay，Transwell assay and ELISA assayResults:The FLS isolated in vitro has a good growth status and possesses the morphological characteristics of fibroblasts; the selected drug concentration range had little effect on the proliferation of FLSCompared with the blank group，higher and lower concentrations of SHC have statistical effects on the proliferation of FLS of higher and lower concentration(P<005)The proliferation of FLS was concentration-dependent with SHC; the migration of OA-FLS can be observed under the microscope，and its migration speed was slowerThe migration rate of 48 h and 72 h in the control group was statistically significant compared with 0 h(P<005)，the 72 h migration rate of the SHC group was statistically significant compared with 0 h(P<005)Compared with the control group，the difference in FLS migration ability of the SHC group at 48 h and 72 h was statistically significant(P<005); The number of cells that FLS invaded and penetrated the compartment membrane in the SHC group was significantly less than that in the blank group(P<005); each concentration group of SHC inhibited the release of MMP3 to varying degrees(P<005)Conclusion:SHC regulates FLS proliferation，inhibits the migration and invasion of FLS，maintains and repairs the integrity of the tissue in the OA joint，and provides new prevention and treatment strategies for the joint protection of OA. |
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