世界中医药

目的：探讨丹七片对缺血性心脏病大鼠模型能量代谢的调节作用。方法：通过结扎左前降支冠状动脉建立缺血性心脏大鼠模型，将48只大鼠分为假手术组、模型组、丹七片组和曲美他嗪组，每组12只。丹七片组大鼠以5000 mg/kg的剂量灌胃丹七片溶液，曲美他嗪组大鼠以60 mg/kg的剂量灌胃曲美他嗪溶液，其他组大鼠灌胃生理盐水。治疗1个月后评价各组大鼠的心脏功能、心肌组织和细胞病理改变、心肌ATP水平及AMPK/SIRT1/PGC-1α信号通路及其下游分子表达水平。结果：超声心动图显示，与模型组比较，丹七片组大鼠的LVIDs和LVIDd分别减少了2635%和2073%，而EF和FS分别增加了3161%和4282%，差异有统计学意义（P<005）。HE染色结果显示，丹七片组的心肌细胞排列较为整齐，细胞水肿减轻，细胞间隙减小，炎性细胞减少。与模型组比较，丹七片组大鼠的心肌ATP水平显著增加（P<005）。免疫组织化学染色结果显示，与模型组比较，丹七片组大鼠PGC-1α的阳性染色评分显著升高（P<005）。Western Blotting结果显示，与模型组比较，丹七片组大鼠的AMPK、SIRT1、PGC-1α、MFN1、MFN2和SOD2蛋白表达均显著升高（P<005）。结论：丹七片可以在缺血性心脏病大鼠模型中发挥心脏保护功能，并上调ATP的产生。丹七片可激活AMPK/SIRT1/PGC-1α信号通路及其下游分子，改善能量代谢并还调节线粒体的生物合成，从而改善大鼠心脏功能并防止心肌损伤。

English Summary:

To investigate the regulation of energy metabolism with Danqi Tablet in a rat model of ischemic heart diseaseMethods:A rat model of ischemic heart was established by ligation of the left anterior descending coronary arteryA total of 48 rats were divided into a sham an operation group，a Danqi tablet group and trimetazidine group，with 12 rats in each groupRats in the Danqi Tablets were given a solution of Danqi Tablets at a dose of 5000 mg/kgRats in the trimetazidine group were given a solution of trimetazidine at a dose of 60 mg/kgThe other groups were given normal salineAfter 1 month of treatment，cardiac function，myocardial tissue cytopathological changes，myocardial ATP levels，and AMPK/SIRT1/PGC-1α signaling pathway and downstream molecular expression were evaluatedResults:Echocardiography showed that compared with the model group，LVIDs and LVIDd in the Danqi tablet group were reduced by 2635% and 2073%，while EF and FS increased by 3161% and 4282%，and the difference was significant(P<005)The results of HE staining showed that the cardiomyocytes in the Danqi tablet group were arranged neatly，the cell edema was reduced，the cell gap was reduced，and the inflammatory cells were decreasedCompared with the model group，the myocardial ATP level of the Danqi tablet group increased significantly(P<005).Immunohistochemical staining showed that the PGC-1α positive staining score of rats in the Danqipian group was significantly higher than that of the model group(P<005)Western Blotting showed that the expression levels of AMPK，SIRT1，PGC-1α，MFN1，MFN2 and SOD2 were significantly increased in the Danqi Tablets group compared with the model group(P<005)Conclusion:Danqi Tablet can play a cardioprotective function in the rat model of ischemic heart disease and up-regulate ATP productionDanqi Tablets activate the AMPK/SIRT1/PGC-1α signaling pathway and its downstream molecules，improve energy metabolism and also regulate mitochondrial biogenesis，thereby improving rat cardiac function and preventing myocardial damage.

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