| 引用本文:王迪1,刘路路1,史学文2.苦参-地榆治疗溃疡性结肠炎活性成分和潜在靶点的网络药理学[J].世界中医药,2021,(16):. |
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| 苦参-地榆治疗溃疡性结肠炎活性成分和潜在靶点的网络药理学 |
| Evaluation of the Active Ingredients and Potential Targets of Radix Sophorae Flavescentis-Radix Sanguisorbae in the Treatment of Ulcerative Colitis with a Network Pharmacology Approach |
| 投稿时间：2020-05-24 |
| DOI：10.3969/j.issn.1673-7202.2021.16.007 |
| 中文关键词: 网络药理学 苦参 地榆 溃疡性结肠炎 作用机制 成分 靶点 通路 |
| English Keywords:Network pharmacology Radix Sophorae Flavescentis Radix Sanguisorbae Ulcerative colitis Mechanism of action Component Target Pathway |
| 基金项目:山东省中医药科技发展计划项目（2019-0187） |
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| 中文摘要: |
| 目的：基于网络药理学预测苦参-地榆治疗溃疡性结肠炎（UC）的作用机制。方法：通过中药系统药理学数据库与分析平台（TCMSP)中药数据库检索苦参-地榆药对的化学成分和靶基因，以口服生物利用度（OB）＞30%和类药性（DL）＞0.18为条件筛选药对的潜在有效成分，并通过TCMSP预测药对的潜在靶点。运用Uniprot数据库寻找到潜在靶点相对应的人类基因名称，通过Genecards、OMIM数据库检索溃疡性结肠炎的疾病靶点，与药对的潜在靶点相映射，绘制韦恩图，将映射找到的有效成分的靶基因导入Cytoscape3.7.2构建药物-成分-疾病-靶点网络图。利用STRING数据库构建蛋白质-蛋白质相互作用（PPI）网络图选出核心靶标，最后将苦参-地榆药对治疗溃疡性结肠炎的有效靶点进行基因本体（GO）富集分析和京都基因和基因组百科全书（KEGG）富集分析。结果：共获得苦参-地榆药对27个有效成分和74个有效靶点。GO富集分析结果显示90个生物过程，主要与DNA结合和蛋白质或辅助因子结合等相关；KEGG通路富集结果显示115条通路，主要包括PI3K-AKT信号通路、癌症通路等。结论：苦参-地榆药对多种活性成分作用于多靶点、多途径、多通路协同发挥治疗UC。 |
| English Summary: |
| To predict the mechanism of treatment of ulcerative colitis(UC)with Radix Sophorae Flavescentis-Radix Sanguisorbae Based on network pharmacology.Methods:Chemical composition and target genes of the Radix Sophorae Flavescentis-Radix Sanguisorbae drug pair were searched through the TCMSP database of traditional Chinese medicines(TCM)，and the potential active ingredients of the drug pair were screened under the condition of bioavailability(OB)>30% and drug-like properties(DL)>0.18.The TCMSP database was used to predict potential targets for the drug pair.Uniprot database was used to find the name of the human gene corresponding to the potential target.The disease target of UC were retrieved through Genecards and OMIM database，and was then mapped to the potential target of the drug pair into a Wayne diagram.The target gene of the component is introduced into Cytoscape 3.7.2 to construct the drug-component-disease-target network diagram.The STRING database was used to construct a PPI protein interaction network diagram to select the core target.Finally，the Radix Sophorae Flavescentis-Radix Sanguisorbae drug pair was used for GO analysis and KEGG analysis of effective targets for the treatment of ulcerative colitis.Results:A total of 27 active ingredients and 74 effective targets were obtained from the Radix Sophorae Flavescentis-Radix Sanguisorbae drug pair.GO enrichment analysis results showed a total of 90 biological processes，mainly related to DNA binding and protein or cofactor binding; KEGG pathway enrichment results showed totally 115 pathways，including PI3K-AKT signaling pathway and cancer pathway.Conclusion:Radix Sophorae Flavescentis-Radix Sanguisorbae drug pair can act on multiple targets，multiple channels，and multiple pathways to treat UC in a variety of active ingredients.This study provides a theoretical reference for further research on the mechanism of action of Radix Sophorae Flavescentis-Radix Sanguisorbae drug pair on UC clinical treatment. |
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