To predict the mechanism of treatment of ulcerative colitis(UC)with Radix Sophorae Flavescentis-Radix Sanguisorbae Based on network pharmacology.Methods:Chemical composition and target genes of the Radix Sophorae Flavescentis-Radix Sanguisorbae drug pair were searched through the TCMSP database of traditional Chinese medicines(TCM)，and the potential active ingredients of the drug pair were screened under the condition of bioavailability(OB)>30% and drug-like properties(DL)>0.18.The TCMSP database was used to predict potential targets for the drug pair.Uniprot database was used to find the name of the human gene corresponding to the potential target.The disease target of UC were retrieved through Genecards and OMIM database，and was then mapped to the potential target of the drug pair into a Wayne diagram.The target gene of the component is introduced into Cytoscape 3.7.2 to construct the drug-component-disease-target network diagram.The STRING database was used to construct a PPI protein interaction network diagram to select the core target.Finally，the Radix Sophorae Flavescentis-Radix Sanguisorbae drug pair was used for GO analysis and KEGG analysis of effective targets for the treatment of ulcerative colitis.Results:A total of 27 active ingredients and 74 effective targets were obtained from the Radix Sophorae Flavescentis-Radix Sanguisorbae drug pair.GO enrichment analysis results showed a total of 90 biological processes，mainly related to DNA binding and protein or cofactor binding; KEGG pathway enrichment results showed totally 115 pathways，including PI3K-AKT signaling pathway and cancer pathway.Conclusion:Radix Sophorae Flavescentis-Radix Sanguisorbae drug pair can act on multiple targets，multiple channels，and multiple pathways to treat UC in a variety of active ingredients.This study provides a theoretical reference for further research on the mechanism of action of Radix Sophorae Flavescentis-Radix Sanguisorbae drug pair on UC clinical treatment.