| 引用本文:陈两绵1，邹芳艳1，2，刘晓谦1，高慧敏1，张永欣1，冯伟红1，郭中原1，王智民1.3种检测方法比较忍冬药用和非药用部位的抗氧化活性[J].世界中医药,2021,(17):. |
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| 3种检测方法比较忍冬药用和非药用部位的抗氧化活性 |
| Comparison of Antioxidant Activities between the Medicinal Parts and Non-Medicinal Parts of Lonicera japonica by Three Test Methods |
| 投稿时间：2021-08-06 |
| DOI：10.3969/j.issn.1673-7202.2021.17.001 |
| 中文关键词: 忍冬 非药用部位 忍冬叶 1,1-二苯基-2-三硝基苯肼 2,2'-联氮双(3-乙基苯并噻唑啉-6-磺酸)二铵盐 铁离子还原能力 抗氧化活性 微量法 |
| English Keywords:Lonicera japonica Non-medicinal parts Lonicerae Japonicae Folium DPPH ABTS FRAP Antioxidant activity Micro-model |
| 基金项目:国家重点研发计划“中医药现代化研究”重点专项(2017YFC1701900);中医药行业科研专项(201507002);中国中医科学院自主选题项目(ZZ0808016,ZZ11-095) |
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| 中文摘要: |
| 目的:分析和比较忍冬药用部位(金银花和忍冬藤)和非药用部位(忍冬叶)的体外抗氧化活性,为忍冬非药用部位——忍冬叶的综合利用和产品开发提供科学依据。方法:分别采用1,1-二苯基-2-三硝基苯肼(DPPH)法、2,2'-联氮双(3-乙基苯并噻唑啉-6-磺酸)二铵盐(ABTS)法和铁离子还原能力(FRAP)微量法测定45份金银花、忍冬叶和忍冬藤样品的抗氧化活性,并分别采用描述性统计、直观分析、系统聚类分析(HCA)和偏最小二乘法判别分析(PLS-DA)等统计学方法进行数据处理和挖掘。结果:3种方法测定的抗氧化活性结果基本相似,测定结果具有较高的可信度。不同产地的金银花、忍冬叶和忍冬藤抗氧化活性大小:连云港≥河南＞山东(金银花);连云港＞河南＞山东(忍冬叶);河南≥山东≥连云港(忍冬藤)。忍冬藤的抗氧化活性较弱,金银花和忍冬叶的抗氧化活性较强。金银花和忍冬叶的抗氧化活性大小因其产地不同而存在差异:忍冬叶≈金银花(河南),忍冬叶＞金银花(连云港),忍冬叶＜金银花(山东)。HCA和PLS-DA分析将45份样品分为2个组别,其中忍冬藤为一组,金银花和忍冬叶为另一组。忍冬藤的抗氧化活性明显不同于金银花和忍冬叶,而金银花和忍冬叶的抗氧化活性则较为相近。结论:忍冬植物的非药用部位——忍冬叶资源丰富,其抗氧化活性比忍冬藤强,与金银花相当,极具综合利用价值和产品开发潜能。 |
| English Summary: |
| To analyze and compare the antioxidant activities in vitro between the medicinal parts［Lonicerae Japonicae Flos(LJFs) and Lonicerae Japonicae Caulis(LJC)］and the non-medicinal parts［Lonicerae Japonicae Folium(LJFm)］of Lonicera japonica Thunb and provide a scientific basis for the comprehensive utilization and product development of LJFm-the non-medicinal parts of Lonicera japonica Thunb.Methods:A total of 3 micro-models of 1,1-diphenyl-2-picrylhydrazyl(DPPH)，2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt(ABTS) and ferric reducing antioxidant power(FRAP) were used respectively to determine the antioxidant activities of 45 samples of LJFs，LJFm and LJC.And descriptive statistics，visual analysis，hierarchical cluster analysis(HCA) and partial least squares discrimination analysis(PLS-DA) were used separately for data processing and mining.Results:The antioxidant activities of 45 samples determined by 3 micro-models were basically consistent，which showed that the measurement results had high reliability.The antioxidant activities of the samples from different producing areas:Lianyungang≥Henan＞Shandong(LJFs); Lianyungang＞Henan＞Shandong(LJFm); Henan≥Shandong≥Lianyungang(LJC).Furthermore，the antioxidant activity of LJC was weak，while that of LJFs and LJFm was strong.The antioxidant activities between LJFs and LJFm varied with the different producing areas，for instance，LJFm≈LJFs(Henan)，LJFm＞LJFs(Lianyungang)，and LJFm＜LJFs(Shandong).A total of 45 samples were divided into 2 groups by HCA and PLS-DA analysis，in which LJC was 1 group and LJFs and LJFm were the other group.It demonstrated that the antioxidant activity of LJC was obviously different from that of LJFs and LJFm，while the antioxidant activity of LJFs was similar with that of LJFm.Conclusion:LJFm as the non-medicinal parts of Lonicera japonica Thunb，has rich resources and its antioxidant activity is stronger than that of LJC and comparable to that of LJFs，so it has great comprehensive utilization value and product development potential. |
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