| 引用本文:刘玉欣1，孙志娟1，代荣琴3，陈正立2，梁闯1，范洪1.雷公藤多苷对类风湿关节炎大鼠Ras/Raf1原癌基因表达的影响[J].世界中医药,2021,(17):. |
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| 雷公藤多苷对类风湿关节炎大鼠Ras/Raf1原癌基因表达的影响 |
| Effects of Tripterygium Glycosides on Ras/Raf1 Expression in Rats with Rheumatoid Arthritis |
| 投稿时间：2021-07-06 |
| DOI：10.3969/j.issn.1673-7202.2021.17.011 |
| 中文关键词: 雷公藤多苷 类风湿关节炎 大鼠 滑膜组织 炎症 病理 |
| English Keywords:Tripterygium glycosides Rheumatoid arthritis Rats Synovial tissue Inflammation Pathology |
| 基金项目:国家自然科学基金项目（81673884） |
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| 中文摘要: |
| 目的：探究雷公藤多苷对类风湿关节炎大鼠关节血清及组织Ras/Raf1表达的影响。方法：将60只SD大鼠随机分为对照组、模型组和干预组，每组20只。模型组与干预组建立大鼠风湿关节炎模型，干预组用雷公藤多苷干预，对照组和模型组给予等量生理盐水。以足趾肿胀度为模型标准和疗效判定指标，实验终点通过酶联免疫吸附试验法（ELISA）检测大鼠血清Ras、Raf1以及炎症介质肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）水平，实验终点通过Western Blotting检测大鼠滑膜组织Ras和Raf1蛋白表达量并对大鼠踝关节进行HE染色分析。结果：ELISA结果表明，与对照组比较，模型组血清Ras、Raf1、TNF-α、IL-6显著升高（均P<0.01），与模型组比较，干预组血清Ras、Raf1、TNF-α、IL-6显著降低（均P<0.01）；Western Blotting结果表明：与对照组大鼠比较，模型组大鼠滑膜组织Ras、Raf1显著升高（均P<0.01），与模型组比较，干预组大鼠滑膜组织Ras、Raf1显著降低（均P<0.05）。HE染色表明：与对照组比较，模型组病理总评分升高（P<0.01），与模型组比较，干预组病理总评分下降（P<0.05）。结论：雷公藤多苷可抑制大鼠血清炎症介质释放并降低外周血和关节组织中Ras、Raf1蛋白的表达，起到骨保护作用。 |
| English Summary: |
| To explore the effects of tripterygium glycosides on the expression of Ras/Raf1 in joint serum and tissues of rats with rheumatoid arthritis.Methods:A total of 60 SD rats were randomly divided into a control group(n=20)，a model group(n=20) and an intervention group(n=20).Rat rheumatoid arthritis model was established in model group and intervention group.The intervention group was intervened with tripterygium glycosides，and the control group and model group were given the same amount of normal saline.Take toe swelling as model standard and curative effect judgment index，at the end of the experiment，the rat serum levels of Ras，Raf1，TNF-α and IL-6 were detected by ELISA.At the end of the experiment，the protein expression of Ras and Raf1 in rat synovial tissue was detected by Western Blotting，and the rat ankle joint was analyzed by HE staining.Results:The results of ELISA showed that，compared with the control group，the levels of serum Ras，Raf1，TNF-α and IL-6 in the model group were increased significantly(P<0.01).Compared with the model group，the levels of serum Ras，Raf1，TNF-α and IL-6 in the intervention group were significantly decreased(P<0.01).The results of Western Blotting showed that，compared with the control group，Ras and Raf1 in rat synovial tissue in the model group were significantly increased(P<0.01)，and compared with the model group，Ras and Raf1 in rat synovial tissue in the intervention group were significantly decreased(P<0.01).He staining showed that，compared with the control group，the total score of pathology in the model group increased(P<0.01)，and compared with the model group，the total score of pathology in the intervention group decreased(P<0.05).Conclusion:Tripterygium glycosides can inhibit the release of serum inflammatory factors in rats and reduce the expression of Ras and Raf1 protein in peripheral blood and joint tissue of rats，which plays a role in bone protection. |
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