| 引用本文:迟鹏1，郝庆2，姚艳春1，孙玉兰1，许德颖3，王嘉怡4.胡黄连苷Ⅱ联合曲美替尼对HT-29细胞凋亡和上皮细胞间质转化的影响[J].世界中医药,2021,(17):. |
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| 胡黄连苷Ⅱ联合曲美替尼对HT-29细胞凋亡和上皮细胞间质转化的影响 |
| Effects of Coprininoside Ⅱ Combined with Trametinib in HT-29 Cells Apoptosis and EMT |
| 投稿时间：2021-07-06 |
| DOI：10.3969/j.issn.1673-7202.2021.17.012 |
| 中文关键词: 胡黄连苷Ⅱ 曲美替尼 结直肠癌 miR-4319 增殖 凋亡 上皮间质转化 HT-29细胞 |
| English Keywords:Cuberitin II Trametinib Colorectal cancer miR-4319 Proliferation Apoptosis EMT HT-29 cells |
| 基金项目:国家自然科学基金项目（81770406） |
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| 中文摘要: |
| 目的：研究胡黄连苷Ⅱ联合曲美替尼通过miR-4319对结直肠癌细胞HT-29凋亡和上皮细胞间质转化（EMT）的影响。方法：将结直肠癌细胞HT-29分成6组，分别为对照组、曲美替尼组、胡黄连苷Ⅱ组、联合组、miR-NC抑制组、miR-4319抑制组，n=9。采用CCK-8实验检测细胞增殖，流式细胞术检测细胞凋亡，Western Blotting法检测Vimentin、E-cadherin、Bax、C-Caspase-3蛋白表达，qRT-PCR检测miR-4319表达。比较6个组的检测结果。结果：与对照组比较，曲美替尼组、胡黄连苷Ⅱ组、联合组的细胞成活率和Vimentin的表达均减少（均P<0.05），而凋亡率和Bax、C-Caspase-3、E-cadherin的表达均增加（均P<0.05）；与miR-NC抑制组比较，miR-4319抑制组的细胞成活率、Vimentin的表达均显著增加（均P<0.05），细胞凋亡率和Bax、C-Caspase-3、E-cadherin蛋白表达却显著减少（均P<0.05）；qRT-PCR结果显示，与对照组比较，曲美替尼组、胡黄连苷Ⅱ组细胞中miR-4319的表达增多（均P<0.05）。结论：胡黄连苷Ⅱ联合曲美替尼通过上调miR-4319诱导结直肠癌细胞HT-29凋亡，并抑制细胞EMT。 |
| English Summary: |
| To study the effects of cucurbitacin Ⅱ combined with trametinib on the colorectal cancer cells HT-29 apoptosis and epithelial-mesenchymal transition(EMT) through miR-4319.Methods:Colorectal cancer cells HT-29 were divided into 6 groups，namely the a control group，a trametinib group，a coprininoside II group，a combination group，an miR-NC inhibition group，and an miR-4319 inhibition group(n＝9).In 6 groups，CCK-8 experiment was used to detect cell proliferation，flow cytometry was used to detect cell apoptosis，Western blot was used to detect the protein expression of Vimentin，E-cadherin，Bax，and C-Caspase-3，and the expression of miR-4319 was detected by qRT-PCR.Compare the experimental results of the 6 groups.Results:Compared with the control group，the cell survival rate，and the expression of Vimentin in the trametinib group，the berberine Ⅱ group and the combination group were reduced(P<0.05)，while the apoptosis rate and the expression of Bax，C-Caspase-3，E-cadherin both increased(P<0.05); compared with the miR-NC inhibition group，the cell survival rate and Vimentin expression in the miR-4319 inhibition group were significantly increased(P<0.05).However，the apoptosis rate，the expression of Bax，C-Caspase-3 and E-cadherin were significantly reduced(P<0.05); qRT-PCR results showed that compared with the control group，the expression of miR-4319 in the cells of the trametinib group and berberineⅡgroup increased(P<0.05).Conclusion:The combination of cucurbitacin Ⅱ and trametinib induces the apoptosis and inhibits cell EMT of colorectal cancer cells HT-29 by up-regulating miR-4319. |
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