| 引用本文:张晓梅1，赵玉涛1，王菊美1，赵社芬1，韩坤岭1，李艳霞2，韩雪3，范秀敏4.姜黄素对结直肠癌细胞迁移、侵袭和上皮间质转化的影响及作用机制[J].世界中医药,2021,(17):. |
|
| 姜黄素对结直肠癌细胞迁移、侵袭和上皮间质转化的影响及作用机制 |
| Effects and Mechanism of Curcumin on Cell Migration，Invasion，and Epithelial-Mesenchymal Transformation in Colorectal Cancer Cells |
| 投稿时间：2021-07-06 |
| DOI：10.3969/j.issn.1673-7202.2021.17.014 |
| 中文关键词: 姜黄素 结直肠癌 增殖 迁移 侵袭 上皮-间质转化 |
| English Keywords:Curcumin Colorectal cancer Proliferation Migration Invasion Epithelial-mesenchymal transformation |
| 基金项目:国家自然科学基金项目（81473585） |
|
| 摘要点击次数: 209 |
| 全文下载次数: 0 |
| 中文摘要: |
| 目的：探究姜黄素对结直肠癌细胞增殖、迁移、侵袭以及上皮间质转化的影响。方法：取结直肠癌HCT116细胞系，随机分为对照组和观察组，观察组细胞使用姜黄素50 μg/mL处理，对照组给予等量二甲基亚砜处理；采用噻唑蓝（MTT）法检测细胞增殖；划痕愈合实验检测细胞迁移；Transwell实验检测细胞侵袭；蛋白免疫印迹法（Western Blotting）检测细胞中上皮性钙黏蛋白（E-cadherin）、神经性钙黏蛋白（N-cadherin）、波形蛋白（Vimentin）、磷酸化Jagged 1蛋白（p-JAG1）、磷酸化Notch受体1蛋白（p-Notch1）以及磷酸化Notch受体2蛋白（p-Notch2）表达水平。结果：MTT法检测显示，姜黄素可显著抑制HCT116细胞增殖。划痕愈合实验以及Transwell实验显示姜黄素可显著抑制HCT116细胞迁移及侵袭。Western Blotting实验显示姜黄素可增加E-cadherin蛋白表达水平，降低N-cadherin和Vimentin蛋白以及p-JAG1、p-Notch1、p-Notch2等蛋白的表达水平（均P<0.05）。结论：姜黄素可显著抑制结直肠癌HCT116细胞增殖、迁移、侵袭以及上皮间质转化，其机制可能与JAG1/Notch2通路失活有关。 |
| English Summary: |
| To investigate the effects of curcumin on the proliferation，migration，invasion and epithelial mesenchymal transformation(EMT) of colorectal cancer cells.Methods:HCT116 cell lines of colorectal cancer were taken and randomly divided into a control group and an intervention group.HCT116 cells in intervention group were treated with curcumin 50 Ug/mL，and HCT116 cells in control group were treated with the same amount of dimethyl sulfoxide.The cell proliferation ability was detected by thiazolium blue(MTT) assay.Cell migration ability was detected by scratch healing assay.Cell invasion ability was detected by Transwell assay.Expression levels of epithelial cadherin(E-cadherin)，neuronal cadherin(N-cadherin)，Vimentin，p-Jagged 1(p-JAG1)，p-Notch receptor 1(p-Notch 1)，and p-Notch receptor 2（p-Notch2) were detected by Western Blotting.Results:Detection of MTT assay showed that curcumin could significantly inhibit the proliferation of HCT116 cells.Wounding healing assay and Transwell assay showed that curcumin could significantly inhibit HCT116 cell migration and invasion.Western Blotting experiments showed that curcumin could increase the expression level of E-cadherin protein，and decrease the expression levels of N-cadherin and Vimentin proteins，as well as p-JAG1，p-Notch1，p-Notch2 and other proteins(P<0.05).Conclusion:Curcumin can significantly inhibit the proliferation，migration，invasion and epithelial mesenchymal transformation of colorectal cancer HCT116 cells，and the mechanism may be related to the inactivation of JAG1/Notch2 pathway. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
