| 引用本文:李艺博1，张悦健1，李娟1，童宏选2，卢涛1.四妙勇安汤“异病同治”动脉粥样硬化与血栓闭塞性脉管炎机制研究[J].世界中医药,2021,(18):. |
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| 四妙勇安汤“异病同治”动脉粥样硬化与血栓闭塞性脉管炎机制研究 |
| Investigate the Effects and Potential Mechanism of “Same Treatment for Different Diseases” of Simiao Yong'an Decoction in the Treatment of Atherosclerosis and Thromboangiitis Obliterans |
| 投稿时间：2021-02-23 |
| DOI：10.3969/j.issn.1673-7202.2021.18.002 |
| 中文关键词: 四妙勇安汤 动脉粥样硬化 血栓闭塞性脉管炎；异病同治；潜在作用机制 网络药理学 |
| English Keywords:Simiao Yong'an(SMYA) Decoction Atherosclerosis Thromboangiitis obliterans Treating different diseases with same method Potential mechanism of action Network pharmacology |
| 基金项目:国家自然科学基金项目（52173276）；北京中医药大学卢涛研究启动经费项目(90011451310009) |
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| 中文摘要: |
| 目的：通过生物信息分析手段探讨四妙勇安汤对动脉粥样硬化（AS）与血栓闭塞性脉管炎（TAO）“异病同治”的潜在分子机制。方法：通过数据库挖掘与检索获取四妙勇安汤中4味中药的活性化合物和对应的靶标；通过GeneCards、比较基因组数据库（CTD）以及美国国家生物技术信息中心（NCBI）数据库筛选AS和TAO的关联靶点；运用Venny 2.1，获得药物作用于二病的共有靶点；基于STRING数据库构建共有靶蛋白的蛋白质-蛋白质相互作用（PPI）网络；通过Cytoscape 3.7.2软件构建药物-活性化合物-共有靶点-疾病整体调控网络图。使用Bioconductor生物信息软件包进行基因本体（GO）功能富集分析和京都基因与基因组百科全书（KEGG）通路富集分析。结果：四妙勇安汤与AS、TAO的共有靶点主要为VEGFA、IL6、JUN、TNF、FOS、MAPK8、MMP9、IL10、ICAM1等计47个；筛选出以黄酮类和甾醇类为主的有效活性成分计9个；KEGG通路富集得到的这些靶点共同参与糖尿病并发症AGE-RAGE、TNF、动脉粥样硬化与流体剪切应力、人巨细胞病毒感染、细胞凋亡、T细胞受体等多条通路调节。结论：四妙勇安汤通过抗炎、减少斑块形成、免疫反应、改变血液流变等多条生物学通路发挥药理作用，证实了其多成分、多靶点、多途径的调节特点，预测了四妙勇安汤“异病同治”AS与TAO的潜在分子机制，为实验设计和后续研究提供理论依据。 |
| English Summary: |
| To explore the the common key target and signaling pathway of Simiao Yong'an(SMYA) Decoction in treating atherosclerosis and thromboangiitis obliterans by bioinformatic analysis.Methods:The active compounds and corresponding targets of 4 traditional Chinese medicines from SMYA Decoction were obtained by database mining and retrieval.The associated targets of AS and TAO were screened by GeneCards，CTD and NCBI database.Venny 2.1 was used to obtain the common targets of the two diseases of drug action.The PPI network of common target proteins was constructed through STRING database.Cytoscape 3.7.2 software was used to construct drug-active compound-common target-disease global regulatory network diagrams.The GO classified enrichment analysis and KEGG pathway enrichment analysis were performed using Bioconductor platform.Results:The common targets of SMYA Decoction in As and Tao were mainly 47，including VEGFA，IL6，Jun，TNF，FOS，MAPK8，MMP9，IL10，ICAM1，etc.A total of 9 active components，mainly flavonoids and sterols，were screened out.These targets were enriched by KEGG pathway and participate in the regulation of multiple pathways such as AGE-RAGE signaling pathway in diabetic complications，TNF signaling pathway，fluid shear stress and atherosclerosis，human cytomegalovirus infection，apoptosis，T cell receptor signaling pathway.Conclusion:The pharmacological effects of SMYA Decoction through anti-inflammation，reducing plaque formation and immune response，changing blood flow changes and other biological pathways have confirmed its multi-component，multi-target and multi-pathway regulatory characteristics.Simultaneously，the study has predicted the potential molecular mechanism for the “same treatment for different diseases” of AS and TAO，and provided theoretical basis for experimental design and further research in traditional Chinese medicine. |
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